中国药物警戒 ›› 2011, Vol. 8 ›› Issue (11): 645-647.

• 基础及临床研究 • 上一篇    下一篇

柴胡总皂苷精制品大鼠肝毒性病理学研究

李晓宇1, 2, 孙蓉1, *, 吕丽莉1, 黄伟1, 钱晓路2, 李素君2, 陆永辉1, 翟丽屏3   

  1. 1 山东省中医药研究院,山东 济南 250014;
    2 山东中医药大学,山东 济南 250355;
    3 山东省地方病防治研究所,山东 济南 250014
  • 收稿日期:2011-07-12 出版日期:2011-11-10 发布日期:2015-07-30
  • 通讯作者: 孙蓉,硕士生导师,研究员,博士后,中药药理与毒理。Email：sunrong107@163.com
  • 作者简介:李晓宇,女,硕士研究生,中药药理与毒理。
  • 基金资助:
    国家自然科学基金项目（30672649）,（81073148）; 山东省科技平台建设项目(2008GG2NS02021); 山东省国际合作引智项目（L20083700336）

Experimental Study on the Pathology of Hepatotoxicity Induced by Saikosaponins in Rats

LI Xiao-yu1, 2, SUN Rong1, *, LV Li-li1, HUANG Wei1, QIAN Xiao lu2, LI Su-jun2, LU Yong-hui1, ZHAI Li-ping3   

  1. 1 Shandong Academy of Chinese Medicine, Shandong Jinan 250014, China;
    2 Shandong University of Traditional Chinese Medicine,Shandong Jinan 250355, China;
    3 Shandong Province Hospital of Traditional Chinese Medicine, Shandong Jinan 250012, China
  • Received:2011-07-12 Online:2011-11-10 Published:2015-07-30

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摘要： 目的 观察连续15 d给予大鼠不同剂量的柴胡总皂苷醇洗脱精制样品导致大鼠肝毒性病理学损伤程度。方法 分别给大鼠灌胃高、中、低剂量的柴胡总皂苷醇洗脱精制样品,按柴胡总皂苷计算,高、中、低剂量组分别为300,150,50 mg·kg-1,除观察一般状况外,剖杀大鼠,精密称取心、肝、脾、肺、肾脏重量,计算脏体比值;进行肝脏病理组织学检查。结果 不同剂量的柴胡总皂苷醇洗脱精制样品可导致大鼠体重下降,肝脏重量和肝体比值增大,病理学检查可见不同程度的肝组织损伤;上述变化随剂量的增加而逐渐加重,与空白组比较有明显差异。结论 柴胡对大鼠肝毒性损伤程度与柴胡用药剂量、柴胡总皂苷含量呈剂量依赖关系。柴胡总皂苷是柴胡导致肝毒性的毒效部位,柴胡总皂苷醇洗脱精制品（81.9%）在一定剂量下,连续给药15d即可导致大鼠明显的肝脏器质性病变,其细胞损伤乃至坏死是主要病理改变。

关键词: 柴胡总皂苷, 醇洗脱, 大鼠, 肝毒性, 病理学

Abstract: Objective The hepatotoxicity induced was studied after given multiple administration of Saikosaponins to mice. Methods The rats were divided into different groups of high, medium and low dose. besides the normal conditions were observed, the heart、 liver、 spleen、 lung、 kidney weigh and volume ratio were calculated, the changes of liver morphology was observed after administration. Results The liver weight and volume ratio was increased, while the rat's weight were decreased. Pathological examination showed different degree of liver pathology tissue damage, and the damage of liver in rats show an obvious dosage relationship. Conclusion the damage of liver show a relationship with the dose of Bupleurum chinense. Saikosaponins is the toxicity site, and this show a organic disease of liver after given Saikosaponins 15 days, the damage and necrosis were the main pathological changes.

Key words: Saikosaponins, alcohol elution, rats, liver toxicity, pathology

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