中国药物警戒 ›› 2015, Vol. 12 ›› Issue (1): 16-19.

• 吴茱萸毒性研究专栏 • 上一篇    下一篇

吴茱萸挥发油多次给药致小鼠肝毒性氧化损伤机制研究

李晓宇1,吴晓文1,窦立雯2,尹利顺2,黄伟1,孙蓉1*   

  1. 1山东省中医药研究院，山东 济南 250014
    2山东中医药大学，山东 济南 250355
  • 收稿日期:2014-12-23 出版日期:2015-01-08 发布日期:2015-07-28
  • 通讯作者: 孙蓉，女，博士，研究员?博导，中药药理与毒理
  • 作者简介:李晓宇，女，在读硕士，中药药理与毒理。
  • 基金资助:
    国家重点基础研究发展计划 （973）中医基础理论专项资助项目 （2009CB522802）；山东省科技平台建设项目课题（2008GG2NS02021）；山东省中医药科技发展计划项目（2013-120）；山东省自主创新和成果转化项目：药物安全性评价和适宜于抗肿瘤及缺血性脑血管疾病多靶点和复方新药成药性评价关键技术研究(2014ZZCX02104)。

Research of Oxidative Damage Mechanism on Hepatotoxicity Caused by Volatile Oil from Evodia Fructus in Mice

LI Xiao-yu1,WU Xiao-wen1,DOU Li-wen1,YIN Li-shun2,HUANG Wei1,SUN Rong1*   

  1. 1Shandong Academy of Chinese Medicine, Shandong Jinan 250014, China;
    2Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China
  • Received:2014-12-23 Online:2015-01-08 Published:2015-07-28

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摘要： 目的 观察连续给予吴茱萸挥发油导致小鼠肝毒性损伤程度与氧化损伤的相关性。方法 小鼠按照吴茱萸挥发油高、中、低剂量分别为1.25、1.0、0.25 mL·kg-1，连续给药7天，每天灌胃给药1次，7天后检测血和肝组织内丙二醛（MDA）水平和超氧化物歧化酶 （SOD） 活性，谷胱甘肽 （GSH）、一氧化氮（NO）含量和谷胱甘肽过氧化物酶（GSH-Px）、一氧化氮合酶（NOS）的活性。结果 吴茱萸挥发油可致血和肝组织内MDA含量增加，同时SOD活性下降；血和肝组织中一氧化氮 （NO） 含量增加，一氧化氮合酶 （NOS） 活性升高；血和肝组织中谷胱甘肽（GSH）含量下降，谷胱甘肽过氧化物酶（GSH-Px）活性下降。上述变化趋势随剂量增加而加重，与空白对照组比较有明显差异。结论 吴茱萸挥发油多次给药后可导致小鼠肝毒性损伤，其损伤途径与引起机体氧化应激后诱导脂质过氧化有关。

关键词: 吴茱萸, 挥发油, 肝毒性, 氧化损伤

Abstract: Objective To investigate the relationship of oxidative damage mechanism on hepatic toxic injury caused by volatile oil from Evodia Fructus in mice. Methods Mice were administrated with volatile oil from Evodia Fructus for 7 days, and the high, middle and low dose groups were separately set 1.25, 1.0, 0.25 mL·kg-1. The level of MDA、SOD、NO、NOS、GSH、GSH-Px in serum and hepatic tissue were detected after administration of 7 d. Results The volatile oil from Evodia Fructus could cause the increased activity of MDA in serum and liver, reduced level of SOD, however, the level of NO and NOS were increased. In addition to the reduced activity of GSH and GSH-Px, all the changes were aggravated in accordance with the dosages. Conclusion The continuous administration of volatile oil from Evodia Fructus on mice can induce obvious hepato toxicity injury. The approach of hepatic damage is related with the peroxidative damage mechanism.

Key words: Evodia Fructus, volatile oil, hepatotoxicity, oxidative damage

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