中国药物警戒 ›› 2017, Vol. 14 ›› Issue (5): 257-262.

• 基础与临床研究专栏 •    下一篇

曼陀罗子对小鼠外周血和骨髓微核及精子畸变的影响

彭晓明, 高莉, 霍仕霞, 闫明*   

  1. 新疆维吾尔自治区维吾尔医药研究所 新疆维吾尔医方剂学实验室,新疆 乌鲁木齐 830049
  • 收稿日期:2017-07-12 修回日期:2017-07-12 出版日期:2017-05-20 发布日期:2017-07-12
  • 通讯作者: 闫 明,男,研究员,药物分析与新药研发。E-mail:yanming21cn@sohu.com
  • 基金资助:
    新疆维吾尔自治区中医民族医药人才培养计划项目（Q2015-03-05）

Study on Micronucleus in Bone Marrow and Peripheral Blood and Sperm Abnormality of Mice Induced by Semen Daturae

PENG Xiao-ming, GAO Li, HUO Shi-xia, YAN Ming*   

  1. Institute of Xinjiang Traditional Uyghur Medicine,Prescription Laboratory of Xinjiang Traditional Uyghur Medicine,Xinjiang Urumqi 830049, China
  • Received:2017-07-12 Revised:2017-07-12 Online:2017-05-20 Published:2017-07-12

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摘要： 目的 探讨曼陀罗子对小鼠外周血、骨髓嗜多染红细胞微核率和精子畸变的影响。方法 将昆明种小鼠按体重随机分为阴性对照组,阳性对照组,曼陀罗子药粉组和曼陀罗子总碱组。阴性对照组小鼠以0.02 mL·g-1·d-1的0.5% CMC-Na灌胃,阳性对照组以0.02 g·kg-1·d-1的环磷酰胺（CP）灌胃,曼陀罗子药粉组分别以2.5、5和10 g·kg-1·d-1的曼陀罗子药粉灌胃,曼陀罗子总碱组以1.25、2.5和5 g·kg-1·d-1的曼陀罗子总碱提取物灌胃,连续给药14d,每隔2d称重。Giemsa染色法观察小鼠外周血和骨髓嗜多染红细胞微核率,伊红染色法观察小鼠精子畸变率。结果 给药组小鼠的体重增量、睾丸和附睾指数均较对照组显著降低,而骨髓和外周血微核率及精子畸变率显著增加。结论 10 g·kg-1·d-1的曼陀罗子药粉和1.25、2.5、5 g·kg-1·d-1的曼陀罗子总碱提取物对小鼠可能具有遗传毒性。

关键词: 曼陀罗子, 小鼠, 微核, 精子畸变

Abstract: Objective To investigate the influence of semen daturae on micronuclei formation in bone marrow and peripheral blood polychromatic erythrocyte. Methods KM strain mice were randomly divided into negative control group, positive control group, semen daturae powder group and total alkaloids of semen daturae group. The negative control group mice were treated with 0.5% CMC-Na and positive control group with 0.02 mL·kg-1·d-1 CP. Semen daturae powder group were administered intragastrically by 2.5, 5 and 10 g·kg-1·d-1 of semen daturae powder, and total alkaloids of semen daturae group by 1.25、2.5 and 5 g·kg-1·d-1 of total alkaloids of semen daturae. Mice body weight was measured every three days, and micronucleus in bone marrow and peripheral blood were analyzed by Giemsa staining and sperm abnormality by Eosin staining. Results Compared with negative control group, semen daturae could significantly decrease the weight increments of mice, and increase the micronucleus in bone marrow and peripheral blood and sperm abnormality in mice. Conclusion 10 g·kg-1·d-1 of semen daturae powder and 1.25, 2.5, 5 g·kg-1·d-1 of total alkaloids of semen daturae may have genetoxicity to mice.

Key words: semen daturae, mouse, micronucleus, sperm abnormality

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