中国药物警戒 ›› 2017, Vol. 14 ›› Issue (4): 215-217.

• 质量与工艺研究 • 上一篇    下一篇

炮制对蔓荆子中主要黄酮成分水溶出率的影响

王婷, 唐力英, 郭日新, 许梦莹, 于现阔, 王祝举   

  1. 中国中医科学院中药研究所,北京 100700
  • 收稿日期:2017-07-05 修回日期:2017-07-05 出版日期:2017-04-20 发布日期:2017-07-05
  • 通讯作者: 唐力英,女,硕士,助理研究员,中药学。E-mail: bjtangliying@163.com
  • 作者简介:王婷,女,在读硕士,中药学。
  • 基金资助:
    国家自然科学基金面上项目（81673602）：“逢子必炒”之科学内涵研究

Influence of Processing on the Dissolution Rates of Flavonoids in Viticis Fructus

WANG Ting, TANG Li-ying, GUO Ri-xin, XU Meng-ying, YU Xian-kuo, WANG Zhu-ju   

  1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Received:2017-07-05 Revised:2017-07-05 Online:2017-04-20 Published:2017-07-05

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摘要： 目的 研究炮制对蔓荆子中黄酮类成分含量的影响,探索炮制对蔓荆子中黄酮类成分水溶出率的影响。方法 建立高效液相色谱法,采用Agilent TC-C18色谱柱(4.6 mm × 250 mm,5 μm）,流动相甲醇（A）-1%冰醋酸水（B）梯度洗脱(0~10 min：25%~35% A;10~18 min：35% A;18~45 min：35%~75% A),流速1 mL·min-1,检测波长254 nm,柱温30 ℃。同时测定蔓荆子生品和炮制品中异荭草素、蔓荆子黄素的含量及水溶出率,分析炮制前后变化。结果 上述2个成分的含量,炮制前分别为0.035%、0.066%,炮制后分别为0.033%、0.056%; 异荭草素、蔓荆子黄素炮制前的水溶出率分别为：40.00%、12.12%,炮制后分别为60.61%、14.29%。结论 炮制会增加蔓荆子中异荭草素、蔓荆子黄素的水溶出率。

关键词: 蔓荆子, 炮制, 蔓荆子黄素, 水溶出率

Abstract: Objective To investigate the influence of processing on the content and dissolution rate of flavonoids in Viticis Fructus. Methods HPLC was used to determine the contents of isoorientin and vitexicarpin in Viticis Fructus simultaneously, chromatographic conditions were listed as follows: Agilent TC-C18 column (4.6 mm × 250 mm, 5 μm), mobile phase of methanol (A) -1% acetic acid water (B) for gradient elution (0~10 min, 25%~35% A; 10~18 min, 35% A; 18~45 min, 35%~75% A), flow rate of 1 mL·min-1, detection wavelength at 254 nm, column temperature at 30 ℃. Changes between the raw and processed Viticis Fructus of these contents and dissolution rates were analyzed. Results The contents of these constituents in raw herbs were 0.035%, 0.066%, their contents were 0.033%, 0.056% after being processed. The dissolution rates of isoorientin and vitexicarpin were 40.0%, 12.1%, and they were 60.6%, 14.3% after being processed. Conclusion Processing would increase the contents and dissolution rates of isoorientin and vitexicarpin in Viticis Fructus.

Key words: Viticis Fructus, processing, vitexicarpin, dissolution rate

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