中国药物警戒 ›› 2013, Vol. 10 ›› Issue (4): 205-206.

• 质量与工艺研究 • 上一篇    下一篇

HPLC法测定黄龙止咳颗粒中盐酸麻黄碱含量

刘红1,田洪根2*   

  1. 1.滨州市产品质量监督检验所,山东滨州256606；
    2.滨州市药品检验所,山东滨州256618
  • 收稿日期:2013-01-04 修回日期:2016-03-09 出版日期:2013-04-08 发布日期:2016-03-09
  • 通讯作者: 田洪根,男,本科,副主任药师,药品检验。E-mail：thggood@163.com
  • 作者简介:刘红,女,本科,质量工程师,产品质量检验。

Determination of Ephedrine Hydrochloride in Huanglong Zhike Granules by HPLC

LIU Hong1 ,TIAN Hong -gen2   

  1. 1.Binzhou Products Quality Supervision and Inspection Institute, Shandong Binzhou 256606, China;
    2.Binzhou Institute for Drug Control, Shandong Binzhou 256618,China
  • Received:2013-01-04 Revised:2016-03-09 Online:2013-04-08 Published:2016-03-09

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摘要： 目的 建立以反相高效液相色谱法测定黄龙止咳颗粒中盐酸麻黄碱含量的方法。方法Agilent Extend C18 色谱柱（250×4.6mm,5μm）,以乙腈-0.1%磷酸溶液（含0.1%三乙胺）（10:90）为流动相,检测波长为207nm。结果 盐酸麻黄碱进样量在0.0700～1.400μg的范围内线性关系良好（r=1.0000）,平均回收率为98.7%。结论本实验方法简单,结果 准确,重现性好,可用于对黄龙止咳颗粒中盐酸麻黄碱含量进行控制。

关键词: 高效液相色谱法, 黄龙止咳颗粒, 盐酸麻黄碱, 含量测定

Abstract: Objective To establish a RP-HPLC method for the determination of ephedrine hydrochloride in Huanglong Zhike granules. Methods Agilent Extend C18 column was used. The mobile phase was acetonitrile-0.1% phosphoric acid(0.1% triethyamine) (10:90). The detection wavelength was set at 207nm. Results A good linearity was obtained in the range of 0.0700μg to 1.400μg(r=1.0000). The average recovery was 98.7%. Conclusion This method is simple, accurate and reproducible, and can be used for the quality control.

Key words: HPLC, Huanglong Zhike granules, ephedrine hydrochloride, determination

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