中国药物警戒 ›› 2012, Vol. 9 ›› Issue (4): 198-201.

• 基础及临床研究 • 上一篇    下一篇

叶下珠提取物对大鼠长期毒性实验研究

戴学栋，戴晓莉，马玉奎   

  1. 山东省医药工业研究所，山东 济南 250033
  • 收稿日期:2011-11-05 出版日期:2012-04-10 发布日期:2015-08-10
  • 作者简介:戴学栋，男，硕士研究生，药师，药物临床前安全性研究。

Experimental Study on the Long-term Toxicity of Extracts of Phyllanthus Urinaria L. in Rats

DAI Xue-dong, DAI Xiao-li, MA Yu-kui   

  1. Shandong Institute of Pharmaceutical Industry, Shandong Jinan 250033, China
  • Received:2011-11-05 Online:2012-04-10 Published:2015-08-10

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摘要： 目的研究连续口服给予叶下珠提取物对SD大鼠产生的毒性反应。方法叶下珠提取物4.0、1.3、0.4 g·kg-1（高、中、低剂量）连续灌胃给药26wk，分别于给药13wk、给药26wk及停药后6wk时取部分大鼠进行血液学指标、血液生化学指标、病理组织学检查。结果高剂量组大鼠皮毛无光泽，精神萎靡。与空白对照组比较，高剂量在给药26wk时导致大鼠血液WBC、NE%、ALT、AST、CR明显升高，LY%、PLT、ALB明显降低（P<0.05或P<0.01），停药后6wk时，除NE%、LY%之外，其他指标均恢复正常。高剂量组的大鼠肝脏、肾脏组织出现可逆性病变，中剂量组的大鼠肾脏组织出现可逆性病变。结论长期大剂量给予叶下珠提取物，可导致SD大鼠出现肝脏和肾脏功能以及病理组织学可逆性改变。

关键词: 叶下珠, 大鼠, 长期毒性

Abstract: Objective To observe the long-term toxicity of the extracts of Phyllanthus urinaria L. in rats by repeated oral administration. MethodsDaily doses of extracts at 4.0 g·kg-1, 1.3 g·kg-1 and 0.5 g·kg-1 for 26 weeks were used for high, mid, and low dose group, respectively. Examinations including haematological indexes, biochemical indexes, and histopathology were performed at 13 weeks, 26 weeks during the administration, and 6 weeks after the withdrawal, respectively. ResultsThe rats in the group apperanced depressed and the fur lack luster during the administration. The levels of WBC, NE%, ALT, AST, and CR in high group were significantly higher than those in control group, and the levels of LY%, PLT, ALB in high group were significantly lower than those in control group at 26 weeks after administration(P <0.05 or P <0.01). Those changed indexes of hematology and blood biochemistery regained normal except NE% and LY% at 6 weeks after withdrawal. The liver of rats in high group and kidney of rats in high and mid group appeared reversible pathological changes. ConclusionRepeated oral administration of the extracts of Phyllanthus urinaria L. at high dose could lead to reversible damage to liver and kidney function and pathology.

Key words: Phyllanthus Urinaria L., rat, long-term toxicity