中国药物警戒 ›› 2012, Vol. 9 ›› Issue (12): 721-724.

• 专栏 • 上一篇    下一篇

北豆根不同组分致小鼠肝毒性与氧化损伤相关性研究

张亚囡1, 2, 罗栋2, 孙蓉1*   

  1. 1 山东省中医药研究院,山东 济南 250014；
    2 山东中医药大学,山东 济南 250355
  • 收稿日期:2012-10-22 出版日期:2012-12-10 发布日期:2015-08-07
  • 通讯作者: 孙蓉,女,博士,研究员,博士生导师,中药药理与毒理。E-mail： sunrong107@163.com
  • 作者简介:张亚囡,女,研究生,中药药理与毒理研究。
  • 基金资助:
    有毒中药的毒性与功效、证候关系的基础研究,课题编号：2009CB522802

The Research of Oxidative Damage Mechanism in Hepatic Toxicity Injury Caused by Different Components from Rhizoma Menispermi in Mice

ZHANG Ya-nan1, 2, LUO Dong2, SUN Rong1*   

  1. 1 Shandong Research Academy of TCM, Shandong Jinan 250014, China;
    2 Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China
  • Received:2012-10-22 Online:2012-12-10 Published:2015-08-07

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摘要： 目的观察连续、较高剂量给予北豆根水提组分和醇提组分导致小鼠肝毒性损伤程度与氧化损伤的相关性。方法小鼠按照北豆根水提组分高、中、低剂量分别为7.2,4.7,1.2 g ·kg-1,醇提组分高、中、低剂量组分别为 26.1,4.7,1.2 g ·kg-1连续给药7天,每天灌胃给药1次,7天后检测血和肝组织内MDA水平和SOD活性,谷胱甘肽（GSH）、一氧化氮（NO）含量和谷胱甘肽过氧化物酶（GSH-Px）、一氧化氮合酶（NOS）的活性。结果北豆根水提组分和醇提组分可致血中和肝组织内MDA含量增加,同时SOD活性下降;血和肝组织中一氧化氮（NO）含量增加,一氧化氮合酶（NOS）活性升高;血和肝组织中谷胱甘肽（GSH）含量下降,谷胱甘肽过氧化物酶（GSH-Px）活性下降。上述变化趋势随剂量增加而加重,与空白对照组有明显差异。结论北豆根水提组分和醇提组分多次给药后可导致小鼠肝毒性损伤,其损伤途径与引起机体氧化应激后诱导脂质过氧化有关。

关键词: 北豆根, 不同组分, 肝毒性, 氧化损伤

Abstract: Objective To investigate the relationship of oxidative damage mechanism in hepatic toxicity caused by different components from Rhizoma Menispermi in mice. Methods Mice were administrated with water extracted and alcohol extracted components for 7 days. The level of MDA、SOD、NO、NOS、GSH、GSH-Px in serum and hepatic tissue were detected. Results The water extracted and alcohol extracted components can cause the activity of MDA in serum and liver increased, the level of SOD corresponding induced, however the level of NO and NOS increased. In addition the activity of GSH and GSH-Px reduced. All the changes were aggravated in accordance with the dosages. Conclusion The continuous administration of water extracted and alcohol extracted components from Rhizoma Menispermi to mice can induce obvious hepato-toxicity injury; the approach of hepatic damage is related with the peroxidative damage mechanism.

Key words: Rhizoma Menispermi, water extracted and alcohol extracted components, hepatotoxicity, oxidative damage mechanism