中国药物警戒 ›› 2011, Vol. 8 ›› Issue (7): 401-406.

• 艾叶毒性与毒靶器官研究 • 上一篇    下一篇

艾叶不同组分对正常大鼠长期毒性实验研究

龚彦胜1, 黄伟2, 钱晓路2, 王会2, 张勇3, 孙蓉1, *   

  1. 1.山东省中医药研究院,山东 济南 250014;
    2.山东中医药大学,山东 济南 250355;
    3.山东省中医院,山东 济南 250012
  • 收稿日期:2010-10-11 出版日期:2011-07-10 发布日期:2015-07-13
  • 通讯作者: *通讯作者：孙蓉,女, 研究员,硕士生导师,中药药理与毒理。E-mail：sunrong107@163.com
  • 作者简介:龚彦胜,男,助理研究员,中药药理与毒理。
  • 基金资助:
    国家重点基础研究发展计划 （973）中医基础理论专项资助项目 （2009CB522802）,山东省科技平台建设项目课题（2008GG2NS02021）

Experimental Study on Chronic Toxicity in Rats Caused by Different Components of Folium Artemisiae Argyi

GONG Yan-sheng1, HUANG Wei2, QIAN Xiao-lu2, WANG Hui2, ZHANG Yong3, SUN Rong1, *   

  1. 1.Shandong Research Academy of TCM, Shandong Jinan 250014, China;
    2.Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China;
    3.Shandong Province Hospital of Traditional Chinese Medicine, Shandong Jinan 250012, China
  • Received:2010-10-11 Online:2011-07-10 Published:2015-07-13

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摘要： 目的 观察连续给予艾叶不同组分导致大鼠慢性毒性的损伤表现、程度及可逆性。方法 连续21天分别给140只大鼠灌胃高、中、低剂量的艾叶水提组分和挥发油组分样品,除观察一般状况外,检测血常规、血生化指标,剖杀大鼠,精密称取心、肝、脾、肺、肾脏等重要脏器,计算脏、体比值,进行常规病理检查。停药后,继续20天恢复期观察。结果 连续21天给予不同剂量的艾叶水提组分和挥发油组分样品均可导致大鼠体重下降,饮食、饮水不佳,血ALT、AST、AKP、TPC增高,ALB降低、A/G比值降低,肝脏重量和肝体比值增大,病理检查可见不同程度的肝脏病理组织损伤;对血常规、肾功能的影响不明显;肝毒性损伤程度与给药剂量呈现一定的剂量依赖相关性;经过20天恢复期观察,上述部分病变不可逆。结论 艾叶水提、挥发油组分对大鼠给药21天导致的长期毒性表现主要是肝损伤,尤其以挥发油的损伤最大,且部分病变为不可逆性损伤。

关键词: 艾叶, 不同组分, 大鼠, 长期毒性

Abstract: Objective The obvious toxic symptoms and signs, the toxic damage extents and reversibility were observed in rats caused by water extract and volatile oil components of Folium Artemisiae Argyi. Methods The 140 rats were divided into seven groups such as normal group、water extract components and volatile oil of high, medium and low dose. The general including the bodyweight、diet and behavior were observed, the blood routine, the biochemical indicators, organ/bodyweight rate, the important organs such as heart、liver、spleen、lung、kidney、brain and et al. and pathological changes were detected after 21 days administrated, and then we continued to observe the 20 days recovery period after drug discontinuance. Results The Wistar rat's weight、diet and drinking were decreased; the serum ALT、AST、AKP、TPC were increased; the ALB and the rate of A/G were decreased; the liver weight and volume ratio were increased after given volatile oil and water extract components from Folium Artemisiae Argyi to Wistar rats by There was little affect for routine blood and renal function; the damage of liver toxicity in rats show an obvious dosage relationship. and after 20 days, the partial lesions were irreversible. Conclusion The main chronic toxical damage is hepatic injury in rats caused by water extract and volatile oil components of Folium Artemisiae Argyi especially the volatile oil, and part of the lesions were irreversible.

Key words: Folium Artemisiae Argyi, different components, rats, chronic toxicity

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