中国药物警戒 ›› 2011, Vol. 8 ›› Issue (4): 193-196.

• 基础研究 •    下一篇

何首乌不同组分单次给药对小鼠肝毒性“量-时-毒”关系研究

黄伟1,2, 张亚囡2, 孙蓉1   

  1. 1. 山东省中医药研究院,山东 济南 250014;
    2. 山东中医药大学,山东 济南 250355
  • 收稿日期:2015-08-27 修回日期:2015-08-27 出版日期:2011-04-10 发布日期:2015-08-27
  • 通讯作者: 孙蓉,女,研究员,硕士生导师,中药药理与毒理。E-mail：sunrong107@163.com
  • 作者简介:黄伟,男,在读硕士研究生,中药药理与毒理。
  • 基金资助:
    山东省科技平台建设项目（2008GG2NS02021）;国家重点基础研究发展计划（973）中医基础理论专项资助项目（2009CB522802）

Experimental Study on the "Dose-Time-Toxicity "Relationship of Acute Hepatotoxicity Induced by Different Components from Polygonum multiflorum in Mice

HUANG Wei1,2, ZHANG Ya-nan2, SUN Rong1   

  1. 1. Shandong Research Academy of TCM, Shandong Jinan 250014, China;
    2. Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China
  • Received:2015-08-27 Revised:2015-08-27 Online:2011-04-10 Published:2015-08-27

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摘要： 目的 观察何首乌不同组分单次给药对小鼠肝毒性“量-时-毒”关系的影响。方法 “时-毒”关系研究：取小鼠按不同时间点分组,单次灌胃给予一定剂量的何首乌水提组分、醇提组分,观察给药后小鼠死亡情况和毒性反应,分别于药后不同时间检测血清丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）,计算肝、脾、胸腺脏器指数。“量-毒”关系研究：取小鼠按不同剂量分组,单次灌胃给予不同剂量的何首乌水提组分、醇提组分,分别于药后4h、2h按“时-毒”研究方法对小鼠进行相应处理。结果 小鼠灌胃较高剂量何首乌水提组分后,血清ALT、AST活力在4h达到高峰,持续时间均约达24h;给药4h后小鼠出现肝脏明显肿大,肝指数升高,其中4~6h肝脏指数升高较为明显。小鼠灌胃较高剂量何首乌醇提组分后,血清ALT、AST活力在2h达到高峰,持续时间均约达48 h;给药2 h后小鼠出现肝脏明显肿大,肝指数升高,其中2~4h肝脏指数升高较为明显。何首乌水提组分剂量在（5.5~30.75）g·kg-1之间、醇提组分在（8.5~24.5）g·kg-1之间对肝组织产生明显损伤,且随着剂量增大,ALT、AST升高显著。结论 小鼠单次灌胃给予一定剂量的何首乌水提组分或醇提组分可造成急性肝损伤,并呈现一定的“量-时-毒”关系。关于其肝脏损伤的机制有待进一步研究。

关键词: 何首乌, 不同组分, 小鼠, 肝毒性, “, 量-时-毒”, 关系

Abstract: Objective To study the "dose-time-toxicity" relationship of hepatotoxicity caused by single dose water extracted and alcohol extracted components of Polygonum multiflorum to mice. Methodse Mice are grouped according to different time or dose points, to observe the death condition and toxicity of mice, detect ALT、AST in serum, calculate liver, spleen, thymus index. Results Compared with the control group, ALT, AST level in serum of water extracted components are peaked after 4 hours' administration, and restored near normality after 24 hours; ALT, AST level in serum of alcohol extracted components are peaked after 2 hours' administration, and restored near normality after 48 hours; The water extracted components of (5.5~30.75)g·kg-1 and alcohol extracted components of (8.5~24.5)g·kg-1 have obvious damage to the liver organization, and ALT, AST in serum increased significantly with the dose increasing. Conclusion The water extracted and alcohol extracted components gavaged certain dose one time to mice could cause obvious acute hepa-injury, and there were certain time-toxicity and dose-toxicity relationships.

Key words: Polygonum multiflorum, water and alcohol extracted components, mice, hepatotoxicity, "dose-time-toxicity" relationship

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