中国药物警戒 ›› 2010, Vol. 7 ›› Issue (9): 524-526.

• 柴胡毒性物质基础研究 • 上一篇    下一篇

柴胡总皂苷粗提取物对大鼠急性毒性实验研究

黄伟12,孙蓉1   

  1. 1.山东省中医药研究院,山东济南 250014；
    2.山东中医药大学,山东济南 250355
  • 收稿日期:2016-03-09 修回日期:2016-03-09 出版日期:2010-09-08 发布日期:2016-03-09
  • 通讯作者: 孙蓉,女,研究员,硕士生导师,中药药理与毒理。E-mail：sunrong107@163.com
  • 作者简介:黄伟,男,在读硕士研究生,中药药理与毒理。
  • 基金资助:
    国家自然科学基金项目（30672649）；山东省科技平台建设项目（2008GG2NS02021）；山东省科技公关关键技术研究课题（2007GG2NS02073）；山东省国际合作引智项目（L20083700336）；国家863科技攻关计划课题（2006GG2202065）；国家重点基础研究发展计划（973）中医基础理论专项资助项目（项目编号：2009CB522802）

Experimental Study on Rat's Acute Toxicity of Total Bupleurum Saponin Extracts

HUANG Wei1,2 ,SUN Rong1   

  1. 1.Shandong Research Academy of TCM, Shandong Jinan 250014, China;
    2.Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China
  • Received:2016-03-09 Revised:2016-03-09 Online:2010-09-08 Published:2016-03-09

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摘要： 目的 探讨柴胡总皂苷粗提取物对大鼠急性毒性的影响。方法 采用经典的急性毒性试验方法,进行柴胡总皂苷粗提取物对大鼠的急性毒性研究。结果 按含柴胡总皂苷含量计算,柴胡总皂苷粗提取物对大鼠的半数致死量（LD50）为2 255.6 mg·kg-1·d-1,95%可信限为2 057.2～2 452.9 mg·kg-1·d-1。死亡大鼠急性毒性主要表现为安静怠动,继而出现步态不稳、腹卧昏睡、心率加快、呼吸急促、连续性抽搐、神经抑制而死亡。存活小鼠在14 天观察期内体重增长缓慢,余未见明显异常。结论 柴胡总皂苷粗提取物可致大鼠全部死亡,且其毒性比小鼠毒性小。其产生毒性的部位及毒性机制有待于深入研究。

关键词: 柴胡总皂苷粗提取物, 大鼠, 急性毒性

Abstract: Objective To discuss the effect of total Bupleurum Saponin extracts on rat's acute toxicity. Methods Make the study on rat's acute toxicity of thetotal Bupleurum Saponin extracts in accordance with classical acute toxicity test methods. Results Caculated by totalsaikosaponin, the LD50 is 2 255.6 mg·kg-1·d-1, and the 95% confidence interval is 2 057.2～2 452.9 mg·kg-1·d-1.The main acute toxicity symptoms of dead rat are negligent action,then arising from unsteady gait, abdominal lying sleeping, rapid heart rate, shortness of breath, continuous convulsions, neurological in hibition, and eventually to death. The surviving rat's weight gainings are slow in 14d observation period and there are no other obvious abnormalities. Conclusion The total Bupleurum Saponin extracts can induce all rats to be dead, and its toxicity on rats is smaller than the mice. The toxical location and mechanism are all to be further studied.

Key words: total Bupleurum Saponin extracts, rats, acute toxicity

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