中国药物警戒 ›› 2010, Vol. 7 ›› Issue (8): 454-456.

• 柴胡毒性影响因素研究 • 上一篇    下一篇

南、北柴胡不同组分对小鼠急性毒性试验比较研究

黄伟1,2, 孙蓉1*, 尹建伟3, 任海勇3   

  1. 1山东省中医药研究院,山东 济南 250014;
    2山东中医药大学,山东 济南 250355;
    3济南杏林生物技术有限公司,山东 济南 250101
  • 收稿日期:2016-02-22 修回日期:2016-02-22 出版日期:2010-08-08 发布日期:2016-02-22
  • 通讯作者: 孙蓉,女,研究员,硕士生导师,中药药理与毒理。E-mail：sunrong107@163.com
  • 作者简介:黄伟, 男,在读硕士研究生,中药药理与毒理。
  • 基金资助:
    国家自然科学基金项目（30672649）; 山东省科技平台建设项目(2008GG2NS02021); 山东省科技公关关键技术研究课题(2007GG2NS02073); 山东省国际合作引智项目（L20083700336）; 国家863科技攻关计划课题（2006GG2202065）; 国家重点基础研究发展计划 （973）中医基础理论专项资助项目 （2009CB522802）

Comparative Study on Mice's Acute Toxicity of Different Components of Radix Bupleuri and Radix Bupleuri Scorzoneraefolii

HUANG Wei1,2, SUN Rong1,*, YIN Jian-wei3, REN Hai-yong3   

  1. 1Shandong Research Academy of TCM, Shandong Jinan 250014, China;
    2Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China;
    3Jinan Xinglin Biology Techonology Corporation, Shandong Jinan 250101, China
  • Received:2016-02-22 Revised:2016-02-22 Online:2010-08-08 Published:2016-02-22

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摘要： 目的 探讨南、北柴胡不同组分对小鼠急性毒性的影响。方法 分别制备南、北柴胡水提组分和醇提组分,按照经典小鼠急性毒性试验方法,进行南、北柴胡不同组分的小鼠急性毒性试验比较研究,试验数据用公斤体重法计算最大耐受量（MTD）和最大给药量（MLD）,连续观察14天。结果 南、北柴胡不同组分对小鼠急性毒性强度为：北柴胡醇提组分>南柴胡醇提组分>北柴胡水提组分>南柴胡水提组分。南、北柴胡水提组分没有出现死亡,测得MLD值按含生药量计算分别为181.6 g·kg-1·d-1和201.6 g·kg-1·d-1,分别相当于临床70 kg人每公斤体重日用量的1 412.4倍和1568.0倍;南、北柴胡醇提组分无法做出LD50,测得MTD值按含生药量计算分别为46.4 g·kg-1·d-1和75.2 g·kg-1·d-1,分别相当于临床70 kg人每公斤体重日用量的360.9倍和584.9倍。结论 北柴胡药材毒性大于南柴胡,且南、北柴胡的醇提组分均大于其水提组分。柴胡皂苷类物质含量高低与其毒性大小有一定相关性,推测柴胡皂苷可能是其主要毒性物质基础。

关键词: 柴胡, 水提组分, 醇提组分, 小鼠, 急性毒性

Abstract: Objective To discuss the effect of different composition of Radix Bupleuri and Radix Bupleuri Scorzoneraefolli on mice's acute toxicity. Methods Respectively prepare the water extracted and alcohol extracted components of Radix Bupleuri and Radix Bupleuri Scorzoneraefolli, and compare the acute toxicity of different components in Radix Bupleuri and Radix Bupleuri Scorzoneraefolli on mice in accordance with classical acute toxicity test Methods, and the experimental datas are calculated MTD and MLD with the Kg body weight method, with the continuous administration and observa-tion for 14 days. Results The acute toxicity of different components in Radix Bupleuri and Radix Bupleuri Scorzoneraefolli on mice is: alcohol extracted components of Radix Bupleuri Scorzoneraefolli >alcohol extracted components of Radix Bupleuri >water extracted components of Radix Bupleuri Scorzoneraefolli >water extracted components of Radix Bupleuri. The water extracted components of Radix Bupleuri and Radix Bupleuri Scorzoneraefolli did not appear dead. Calculated by crude drug content, the MLD were respectively 181.6 g·kg-1·d-1 and 201.6 g·kg-1·d-1,which is equal to 1412.4 times and 1568.0 times of 70kg people's daily dried medicinal herb expenses. The alcohol extracted components of Radix Bupleur and Radix Bupleuri Scorzoneraefolli are unable to make LD50, the MTD were respectively 46.4 g·kg-1·d-1 and 75.2 g·kg-1·d-1,which is equal to 360.9 times and 584.9 times of 70kg people's daily dried medicinal herb expenses. Conclusion The toxicity of Radix Bupleuri Scor-zoneraefolli is bigger than the Radix Bupleur, and the toxicity of the alcohol extracted components is bigger than the water extracted components. There is some relevance between the content of Bupleurum saikosaponin and the toxicity, so we speculated that the Bupleurum saikosaponin may be the mainly toxical basis.

Key words: Radix Bupleuri and Radix Bupleuri Scorzoneraefolli, water extracted components, alcohol extracted components, mice, acute toxicity

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