中国药物警戒 ›› 2010, Vol. 7 ›› Issue (12): 708-709.

• 基础研究 • 上一篇    下一篇

HPLC法同时测定刺五加果实中齐墩果酸和熊果酸的含量

张海丰1,孙健2,滕坤1,朱俊义1   

  1. 1.通化师范学院,吉林通化134000；
    2.中国中医科学院医学实验中心,北京100700
  • 收稿日期:2010-08-15 修回日期:2016-03-09 出版日期:2010-12-08 发布日期:2016-03-09
  • 通讯作者: 朱俊义,男,教授,药用植物的功能饮品开发。E-mail：swx0527@163.com
  • 作者简介:张海丰,男,博士,中药化学与新药基础研究。

Simultaneously Determination of Oleanolic Acid and Ursolic Acid in Medofenoxate Fruits by HPLC

ZHANG Hai-feng1 ,SUN Jian2 ,TENG Kun1 ,ZHU Jun-yi1   

  1. 1.Tonghua Normal University, Jilin Tonghua, 134000, China;
    2.Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Received:2010-08-15 Revised:2016-03-09 Online:2010-12-08 Published:2016-03-09

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摘要： 目的 建立HPLC 同时测定刺五加果实中齐墩果酸和熊果酸含量的方法。方法 采用Agilent XDB-C18色谱柱（4.6mm×250mm,5 m）,以甲醇- 乙腈-0.3%磷酸溶液（30 60 10）为流动相,流速：0.8mL·min-1,柱温为室温,检测波长为210nm。结果 齐墩果酸进样量在0.2052～3.2832 g范围内线性关系良好,r=0.9995,平均加样回收率为101.11%,RSD 为1.69%（n=6）；熊果酸进样量在0.2072～3.3152 g范围内线性关系良好,r=0.9993,平均加样回收率为98.51%,RSD 为2.67%（n=6）。结论 该方法灵敏快速、结果准确、重现性好,可用于刺五加果实中齐墩果酸和熊果酸含量的同时测定。

关键词: 刺五加果实, 齐墩果酸, 熊果酸, 高效液相色谱

Abstract: Objective To establish a HPLC method for the simultaneous determination of Oleanolic Acid and Ursolic Acid in Medofenoxate fruits. Methods Agilent XDBC18(4.6 mm×250 mm,5μm) column was applied, using methanolacetonitrile-0.3% phosphoric acid(30 ：60 ：10) as mobile phase with the rate of 0.8mL·min-1. The column temperature was room temperature and the detective wavelength was 210nm. Results A good linearity was obtained over the range of 0.2052～3.2832μg for Oleanolic Acid(r=0.9995). The average recovery(n=6) was 101.11%(RSD=1.69%);A good linearity was obtained over the range of 0.2072～3.3152μg for Ursolic Acid(r=0.9993). The average recovery(n=6) was 98.51%(RSD=2.67%). Conclusion The method is practical, convenient, accurate, and can be used as the simultaneous determination of Oleanolic Acid and Ursolic Acid in Medofenoxate fruits.

Key words: medofenoxate fruit, oleanolic acid, ursolic acid, HPLC

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