中国药物警戒 ›› 2026, Vol. 23 ›› Issue (10): 1108-1114.
DOI: 10.19803/j.1672-8629.20260257

• 蒙药药效物质基础与作用机制研究专栏（二） • 上一篇    下一篇

蒙药蓝盆花醇提物对小鼠药物性肝损伤的保护作用及机制研究

陈星雨1, 周树宏2,3, 王璐3, 何建祺2, 田金墁4, 包福顺5, 张文雪1, 马奥6, 包丽丽2, 王敏杰2,3#, 伊乐泰1,3,7*   

  1. 1内蒙古医科大学药学院,内蒙古 呼和浩特 010059;
    2内蒙古医科大学基础医学院,内蒙古 呼和浩特 010059;
    3内蒙古医科大学中药（蒙药）质量研究与药效评价重点实验室,内蒙古 呼和浩特 010059;
    4内蒙古医科大学内蒙古临床医学院,内蒙古 呼和浩特 010017;
    5内蒙古医科大学蒙医药学院,内蒙古 呼和浩特 010059;
    6包头医学院药学院,内蒙古 包头 014000;
    7内蒙古医科大学民族医药创新中心,内蒙古 呼和浩特 010110
  • 收稿日期:2026-03-27 出版日期:2026-10-15 发布日期:2026-10-16
  • 通讯作者: *伊乐泰,男,硕士,教授·硕导,中蒙药活性物质基础研究。E-mail: yiletai@126.com。#为共同通信作者。
  • 作者简介:陈星雨,女,在读硕士,中蒙药活性物质基础研究。
  • 基金资助:
    国家自然科学基金资助项目（82560126）;内蒙古自治区中医药（蒙医药）中青年领军人才培育项目[2022-（RC014）];内蒙古医科大学科技创新团队项目（YKD2022TD001）

Hepatoprotective effects and underlying mechanisms of Ethanolic extract of Mongolian medicinal herb Scabiosa comosa on drug-induced liver injury in rats

Chen Xingyu1, Zhou Shuhong2,3, Wang Lu3, He Jianqi2, Tian Jinman4, Bao Fushun5, Zhang Wenxue1, Ma Ao6, Bao Lili2, Wang Minjie2,3#, Yi Letai1,3,7*   

  1. 1School of Pharmacy, Inner Mongolia Medical University, Hohhot Inner Mongolia 010059, China;
    2School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot Inner Mongolia 010059, China;
    3Key Laboratory of Quality Research and Efficacy Evaluation of Traditional Chinese Medicine (Mongolian medicine), Inner Mongolia Medical University, Hohhot Inner Mongolia 010059, China;
    4Clinical Medical College, Inner Mongolia Medical University, Hohhot Inner Mongolia 010017, China;
    5School of Mongolian Medical and Pharmaceutical Science, Inner Mongolia Medical University, Hohhot Inner Mongolia 010059, China;
    6School of Pharmacy, Baotou Medical College, Baotou Inner Mongolia 014000, China;
    7Medical Innovation Center for Nationalities, Inner Mongolia Medical University, Hohhot Inner Mongolia 010110, China
  • Received:2026-03-27 Online:2026-10-15 Published:2026-10-16

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摘要： 目的 探究蒙药蓝盆花（Scabiosa comosa Fisch. ex Roem. & Schult.）醇提物对药物性肝损伤的药效物质基础和可能的机制。方法 预防性给药8周后,建立急性药物性肝损伤C57BL/6小鼠模型,取血清检测肝功能指标丙氨酸氨基转移酶（Alanine Aminotransferase,ALT）、天冬氨酸氨基转移酶（Aspartate Aminotransferase,AST）,取肝脏组织进行病理学染色（HE）及转录组学测序分析。通过网络药理学分析获得蓝盆花醇提物对急性药物性肝损伤可能作用靶点,并对可能的靶点进行mRNA表达水平验证,确定核心靶点,与蓝盆花醇提物入血成分进行分子对接,初步筛选核心成分。结果 血清肝功能指标检测表明,与模型组相比,蓝盆花醇提物中剂量组可显著下调ALT及AST活性,差异具有统计学意义（P<0.05）。病理学染色结果显示,与模型组相比,蓝盆花醇提物低剂量和中剂量组改善情况较好。转录组目的基因集的KEGG通路富集分析和GO功能富集分析表明免疫反应相关生物过程和通路中有显著富集。RT-qPCR结果显示,血管黏附因子（Vascular Cell Adhesion Molecule 1,Vcam1）、趋化因子（C-C基序）配体2（C-C Motif Chemokine Ligand 2,Ccl2）和雄激素受体（Androgen Receptor,Ar）在模型组与蓝盆花醇提物中剂量组之间差异显著（P<0.05）。分子对接表明,Vcam1和木犀草素、Ccl2和异绿原酸A、Ar和绿原酸之间均具有良好的结合性（结合能小于-6 kcal·mol-1）。结论 初步证明蓝盆花醇提物中的木犀草素、异绿原酸A、绿原酸等成分可能通过调控TNF通路相关的下游分子（Vcam1、Ccl2）,缓解炎症反应,保护急性药物性肝损伤。

关键词: 蒙药, 蓝盆花, 药物性肝损伤, 对乙酰氨基酚, 血管黏附因子, 趋化因子（C-C基序）配体2, 雄激素受体, 醇提物, 小鼠

Abstract: Objective To explore the pharmacodynamic material basis and potential mechanism of Mongolian medicine Scabiosa comosa Fisch. ex Roem. & Schult. ethanol extract against drug-induced liver injury (DILI). Methods After 8 weeks of preventive administration, an acute drug-induced liver injury model was established in C57BL/6 mice. Serum samples were collected to detect such liver function indicators as alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Liver tissues were subjected to hematoxylin-eosin (HE) pathological staining and transcriptome sequencing analysis. Network pharmacology was applied to screen potential targets of the ethanolic extract of Scabiosa comosa against acute drug-induced liver injury. The mRNA expression levels of candidate targets were verified to identify core targets, which were subjected to molecular docking with blood-absorbed components of the extract to screen core active ingredients. Results Serum liver function tests found that the activities of ALT and AST were significantly reduced in the medium-dose group of Scabiosa comosa ethanolic extract compared with the model group, with statistically significant differences (P<0.05). Pathological staining suggested that hepatic pathological damage was markedly mitigated in both low- and medium-dose extract groups. KEGG pathway enrichment and GO functional enrichment analyses of target gene sets from the transcriptome data revealed significant enrichment in biological processes and signaling pathways related to immune response. RT-qPCR results indicated that the expression levels of vascular cell adhesion molecule 1 (Vcam1), C-C motif chemokine ligand 2 (Ccl2) and androgen receptor (Ar) were significantly different between the model group and the medium-dose extract group (P<0.05). Molecular docking results showed favorable binding affinities between Vcam1 and luteolin, Ccl2 and isochlorogenic acid A, as well as Ar and chlorogenic acid, with binding energies all lower than -6 kcal·mol-1. Conclusion The active components in the ethanolic extract of Scabiosa comosa, including luteolin, isochlorogenic acid A and chlorogenic acid, could alleviate inflammatory response and ameliorate acute drug-induced liver injury by regulating downstream molecules (Vcam1, Ccl2) involved in the TNF signaling pathway.

Key words: Mongolian Medicine, Scabiosa comosa, Drug-Induced Liver Injury, Acetaminophen, Vascular Cell Adhesion Molecule 1 (Vcam1), C-C Motif Chemokine Ligand 2 (Ccl2), Androgen Receptor (Ar), Ethanol Extract, Mice

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