红参-制何首乌药对抗衰老作用的HT22细胞和秀丽隐杆线虫模型初步研究

doi:10.19803/j.1672-8629.20230111

中国药物警戒 ›› 2023, Vol. 20 ›› Issue (6): 629-633.
DOI: 10.19803/j.1672-8629.20230111

• 何首乌质量安全及药效研究专栏 • 上一篇下一篇

红参-制何首乌药对抗衰老作用的HT22细胞和秀丽隐杆线虫模型初步研究

刘晶晶¹, 徐蓓蕾², 杨建波¹, 王莹¹, 刘越³, 汪祺^1#, 马双成^1,*

¹中国食品药品检定研究院中药民族药检定所,北京 100050;
²哈尔滨商业大学药学院,黑龙江哈尔滨 150000;
³北京中医药大学中药学院,北京 100871

收稿日期:2023-02-27 出版日期:2023-06-15 发布日期:2023-06-15
通讯作者: ^* 马双成,男,研究员·博导,中药民族药物质基础与质量安全评价研究。E-mail：msc@nifdc.org.cn。^#为共同通信作者。
作者简介:刘晶晶,女,硕士,助理研究员,中药质量控制研究。
基金资助:
国家自然科学基金资助项目(81973476)

HT22 Cell and Caenorhabditis elegans models of the anti-aging effect of Ginseng Radix et Rhizoma Rubra and Polygonum Multiflorum Radix preparata medicinal pair

LIU Jingjing¹, XU Beilei², YANG Jianbo¹, WANG Ying¹, LIU Yue³, WANG Qi^1#, MA Shuangcheng^1,*

¹National Institutes for Food and Drug Control, Institute for Control of Chinese Traditional Medicine and Ethnic Medicine, Beijing 100050, China;
²School of Pharmacy, Harbin University of Commerce, Harbin Heilongjiang 150000, China;
³School of Chinese Materia medica, Beijing University of Chinese Medicine, Beijing 100871, China

Received:2023-02-27 Online:2023-06-15 Published:2023-06-15

摘要/Abstract

摘要： 目的初步探讨不同提取工艺下红参-制何首乌药对对神经细胞的抗衰作用,为红参-制何首乌药对在抗衰及抗阿尔茨海默病（AD）作用的应用提供数据支撑。方法分别制备30%和70%乙醇回流提取物,30%和70%乙醇渗漉提取物和水提取物。通过流式细胞术检测不同提取物对HT22细胞中β-半乳糖苷酶含量的影响,初步筛选红参-制何首乌药对神经细胞抗衰效果最佳的提取物,并通过β-半乳糖苷酶染色直观分析该提取物对神经细胞的抗衰效果,用秀丽隐杆线虫寿命试验及瘫痪实验验证其抗衰及抗AD作用。结果红参-制何首乌药对的不同提取方式均对HT22细胞具有显著的抗衰老作用,其中70%乙醇渗漉提取物效果最佳。对其进行多浓度验证,显示具有剂量依赖性的保护作用。浓度为0.2 mg·mL^-1的70%乙醇渗漉提取物对秀丽隐杆线虫的寿命有明显的延长作用,浓度为0.008 mg·mL^-1的70%乙醇渗漉提取物具有缓解瘫痪的趋势。结论红参-制何首乌药对的70%乙醇渗漉提取物具有明显抗神经细胞衰老的作用,具有抗衰老延长寿命与治疗AD的潜力,应深入研究。

关键词: 红参-制何首乌药对, 抗衰老, HT22细胞, 秀丽隐杆线虫, 阿尔默茨海默症, 安全性, 寿命实验, β-半乳糖苷酶

Abstract: Objective To preliminarily discuss the anti-aging effect of Ginseng Radix et Rhizoma Rubra(GR) and Polygoni Multiflori Radix Preparata (PMRP) on nerve cells under different extraction processes, and provide data support for the application of GR and PMRP in anti-aging and anti-Alzheimer's disease (AD). Methods GR and PMRP medicinal pair extracts of 30% ethanol reflux, 30% ethanol percolation, 70% ethanol reflux, 70% ethanol percolation and water extract were prepared. The effects of different extracts on β-Galactosidase content in HT22 cells were detected by flow cytometry, and the extracts with the best anti-aging effect on nerve cells were initially screened. The anti-aging effect of the extracts on nerve cells was directly analyzed by β-galactosidase staining, and the anti-aging and anti-AD effects were verified by nematode life test and paralysis experiment. Results The different extraction processes of GR and PMRP showed significant anti-aging effects on HT22 cells, and 70% ethanol percolation extract had the best effect. Multi-concentration validation showed that it had a dose-dependent protective effect. The 70% ethanol percolation extract at 0.2 mg•mL^-1 could significantly prolong the life of Caenorhabditis elegans, and the 70% ethanol percolate extract at 0.008 mg•mL^-1 showed a tendency to relieve paralysis. Conclusion The 70% ethanol percolation extract of GR and PMRP has obvious anti-aging effect on nerve cells, has the potential of anti-aging and prolonging life and treating AD, which should be further studied.

Key words: Ginseng Radix et Rhizoma Rubra and Polygoni Multiflori Radix Preparata medicinal pair, anti-aging, HT22 cells, Caenorhabditis elegans, Alzheimer's disease, safety, life experiment, beta-galactosidase

中图分类号:

R917

刘晶晶, 徐蓓蕾, 杨建波, 王莹, 刘越, 汪祺, 马双成. 红参-制何首乌药对抗衰老作用的HT22细胞和秀丽隐杆线虫模型初步研究[J]. 中国药物警戒, 2023, 20(6): 629-633.

LIU Jingjing, XU Beilei, YANG Jianbo, WANG Ying, LIU Yue, WANG Qi, MA Shuangcheng. HT22 Cell and Caenorhabditis elegans models of the anti-aging effect of Ginseng Radix et Rhizoma Rubra and Polygonum Multiflorum Radix preparata medicinal pair[J]. Chinese Journal of Pharmacovigilance, 2023, 20(6): 629-633.

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红参-制何首乌药对抗衰老作用的HT22细胞和秀丽隐杆线虫模型初步研究

HT22 Cell and Caenorhabditis elegans models of the anti-aging effect of Ginseng Radix et Rhizoma Rubra and Polygonum Multiflorum Radix preparata medicinal pair

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