中国药物警戒 ›› 2021, Vol. 18 ›› Issue (7): 637-640.
DOI: 10.19803/j.1672-8629.2021.07.09

• 基础与临床研究 • 上一篇    下一篇

一测多评法用于橘核中多成分含量测定的适用性研究

麻朝朝1,2,3, 王倩2,3,4, 高乐2,3,4, 甄亚钦2,3,4, 王鑫国2,3,4, 牛丽颖2,3,4#, 张丽艳1,*   

  1. 1贵州中医药大学,贵州 贵阳550025;
    2河北省中药配方颗粒技术创新中心,河北 石家庄050091;
    3河北省中药材品质评价与标准化工程研究中心,河北 石家庄050091;
    4河北中医学院,河北 石家庄050091
  • 收稿日期:2021-04-08 出版日期:2021-07-15 发布日期:2021-07-23
  • 通讯作者: *张丽艳,女,学士,教授,中药及民族药质量控制及新药研究。E-mail:zly1964@163.com;#为共同通信作者。
  • 作者简介:麻朝朝,女,硕士,中药及民族药质量控制及新药研究。
  • 基金资助:
    中央引导地方科技发展资金项目（206Z2501G）; 河北省重点研发计划项目（20372502D）; 河北省自然科学基金资助项目（H2019423050）

Study on Applicability of QAMS to Multi-component Content Determination of Citri Reticulatae Semen

MA Zhaozhao1,2,3, WANG Qian2,3,4, GAO Le2,3,4, ZHEN Yaqin2,3,4, WANG Xinguo2,3,4, NIU Liying2,3,4#, ZHANG Liyan1,*   

  1. 1Guizhou University of Traditional Chinese Medicine, Guiyang Guizhou 550025, China;
    2Hebei TCM Formula Granule Innovation Center, Shijiazhuang Hebei 050091, China;
    3Hebei TCM Quality Evaluation ＆ Stan-dardization Engineering Research Center, Shijiazhuang Hebei 050091, China;
    4Hebei University of Chinese Medicine, Shijiazhuang Hebei 050091, China
  • Received:2021-04-08 Online:2021-07-15 Published:2021-07-23

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摘要： 目的 建立一测多评法同时测定橘核中柠檬苦素、诺米林、黄柏酮3种成分含量的方法,并验证该法在橘核质量控制中应用的可行性与适用性。方法 橘核含量测定采用高效液相色谱法,以Waters Symmetry C18柱（4.6×250 mm, 5 μm）为色谱柱,流动相为乙腈-水（45∶55）,流速为1 mL/min,柱温35 ℃,检测波长为220 nm。以柠檬苦素为内参物,计算诺米林、黄柏酮的相对校正因子,测定橘核中3种成分的含有量。结果 建立了橘核中柠檬苦素与诺米林、黄柏酮的相对校正因子;3种成分一测多评法的计算值与外标法实测值间无明显差异。结论 一测多评的质量控制模式可用于橘核中3种不同成分的多指标同步质量评价。

关键词: 一测多评, 相对校正因子, 柠檬苦素, 橘核, 质量评价

Abstract: Objective To establish a quantitative analysis of multi-components by single mark (QAMS) for determining the contents of three compositions (limonin, nomilin, obacunone) from orange kernels, salt citri reticulatae semen and their granule, and verify the feasibility and applicability of this method in quality control. Methods Limonin was used as the internal reference substance. The HPLC analysis was performed on a Waters Symmetry C18 (4.6×250 mm, 5 μm) column with a mobile phase consisted of acetonitrile and water (45∶55) at a flow rate of 1 mL/min-1. The column temperature was maintained at 35 ℃, and the detection wavelength was set at 220 nm. Results The relative correction factors of Limonin, nomilin and obacunone were established, without significant difference between the calculated values by QAMS and measured valves. Conclusion The experimental QAMS method can accurately determine the contents of the three compositions in citri reticulatae semen, and can be used in the multi-index evaluation in orange kernels.

Key words: quantitative analysis of multi-compounds by single-marker, relative correction factor, limonin, citri reticulatae semen, quality evaluation

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