Abstract: Cytometric bead array(CBA) method for the detection of secreted cytokines was established and optimized on FACSAriaⅠcytometer. The concentrations of IL-6、IL-10、MCP-1、TNF、IFN- 、IL12p70 cytokines secreted by LPS-stimulated RAW264.7 cells were detected by means of CBA． Berberine can significantly down-regulate secretion of various inflammatory cytokines. The inhibitory rate of 12μmol/L berberine against IL-10, IFN- , TNF, IL-12 p70 were 5.10±0.24% ,18.64±3.95%, 22.59±0.56%, 68.27±2.49%, respectively. Berberine at 48μmol/L remarkably inhibited the increasing expression of IL-10, IFN- , TNF, IL-12 p70 stimulated by LPS by 45.62±1.51%, 39.00±3.58%, 79.61±5.17%, 92.03±0.85%. CBA assay is a realistic high-throughput approach for the detection of inflammatory cytokines because of its sensitivity, efficiency, cost-effectiveness and reproducibility.

Key words: cytometric bead array, cytokines, berberine