Abstract: ObjectiveTo study the "dosage-time-toxicity" relationship of hepatotoxicity induced by single intragastric administration of water extraction from Rhizoma Pinelliae. Methods"time-toxicity" relationship study:preparing water extraction of 62.5 g·kg-1, ig it of 25 mL·kg-1 to mice once,then get their blood sampling after administration at different times(0, 0.5, 1, 2, 4, 8, 12, 24, 48, 72h). The changes of the activity of ALT, AST and hepar index were detected and the histopathological study was performed; kg-1、70.1 g·kg-1、59.6 g·kg-1、50.7 g·kg-1、43.1 g·kg-1、36.6 g·kg-1, ig them to mice once,while ig distilled water to control group, then do the detecting work 4 hours later as above. Results"time-toxicity"relationship study indicates that the activity of serum ALT, AST is changing with the time, its toxic peak appears at 4th hour after administration and last for about 48 hours; "dosage-toxicity" relationship study indicates that the active of serum ALT, AST of high dosage groups were significantly increased than that of normal group.Hepatic tissue pathomorphology examination also shows that hydroncus, fatty degeneration and necrosis in hepatocyte could be induced after administration the high dosage samples,while the low dosage groups have no differences. ConclusionSingle intragastric administration of water extraction from Rhizoma Pinelliae with certain dosage may induce acute hepatotoxical injury in mice and show an obvious "dosage-time-toxicity" relationship.

Key words: Rhizoma Pinelliae, mice, hepatotoxicity, "dosage-time-toxicity", relationship