Abstract: Objective To investigate the influence of semen daturae on micronuclei formation in bone marrow and peripheral blood polychromatic erythrocyte. Methods KM strain mice were randomly divided into negative control group, positive control group, semen daturae powder group and total alkaloids of semen daturae group. The negative control group mice were treated with 0.5% CMC-Na and positive control group with 0.02 mL·kg^-1·d^-1 CP. Semen daturae powder group were administered intragastrically by 2.5, 5 and 10 g·kg^-1·d^-1 of semen daturae powder, and total alkaloids of semen daturae group by 1.25、2.5 and 5 g·kg^-1·d^-1 of total alkaloids of semen daturae. Mice body weight was measured every three days, and micronucleus in bone marrow and peripheral blood were analyzed by Giemsa staining and sperm abnormality by Eosin staining. Results Compared with negative control group, semen daturae could significantly decrease the weight increments of mice, and increase the micronucleus in bone marrow and peripheral blood and sperm abnormality in mice. Conclusion 10 g·kg^-1·d^-1 of semen daturae powder and 1.25, 2.5, 5 g·kg^-1·d^-1 of total alkaloids of semen daturae may have genetoxicity to mice.

Key words: semen daturae, mouse, micronucleus, sperm abnormality