Effect of Neferine on Expression of NF-κB Signaling Pathway and Correlation Factors in LPS-induced Human Umbilical Vein Endothelial Injury

Abstract

Abstract: Objective To observe the anti-atherosclerosis effect of neferine on activiation of NF-κB and correction factors by establishing of VEC-SMC co-culture system induced by LPS. Methods An indirect co-cultivating system between VEC and SMC was constructed by planting VEC in Transwell cell’s upper chamber and SMC in lower chamber. The injuring condition of VEC was built after stimulating VEC using LPS, which was treated with neferine in different concentrations (0, 0.1, 0.3, 1, 3 μmol·L^-1) for 24 h. The protein levels of IκB-ɑ, NF-κB p65 were detected by Western blotting. The mRNA expression of TNF-ɑ and ICAM-1 were determined by RT-PCR. Results After treated with Nef, the expression of NF-κB p65, TNF-ɑ mRNA and ICAM-1 mRNA in HUVECs injured by LPS were decreased, while the expression of IκB-ɑ was increased in a concentration-dependent manner. Conclusion Neferine may inhibit NF-κB signaling pathway and decrease the expression of correction factors, in order to reduce the inflammatory reaction in HUVECs and alleviate the atherosclerosis.

Key words: neferine, LPS, HUVECs, NF-kappa B signaling pathway, inflammatory factor

CLC Number:

DAI Zhun, SONG Jin-chun. Effect of Neferine on Expression of NF-κB Signaling Pathway and Correlation Factors in LPS-induced Human Umbilical Vein Endothelial Injury[J]. Chinese Journal of Pharmacovigilance, 2017, 14(3): 129-132.

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