Abstract: Objective To investigate the repeated dose toxicity of total flavonoids from Rosa Chinensis in Beagle dogs.Methods Forty normal Beagle dogs were divided into four groups: normal control group and 0.9, 0.3, 0.1 g·kg^-1 dose of total flavonoids from Rosa Chinensis group. The dogs were administered intragastrically total flavonoids from Rosa Chinensis for 13 weeks. The body weight, hematology, blood biochemistry, viscera index and histopathology were measured for the overall toxicity assessment.Results The normal dogs administered total flavonoids from Rosa Chinensis (0.9 g·kg^-1) significantly showed abnormal escalation of the levels of creatinine (CR) , urea nitrogen(BUN) and kidney index. Pathological changes of kedney were found by histopathological examination.Conclusion Total flavonoids from Rosa Chinensis (0.9g·kg^-1) administered intragastrically has significant toxicity on kidney.

Key words: total flavonoids from Rosa Chinensis, Beagle dog, repeated dose toxicity, kidney toxicity