Abstract: Objective To establish a HPLC method for simultaneous determination of catalpol,verbascoside,atractylenolide-I and atractylenolide-Ⅲ in Dangshen Yangrong pills. Methods Chromatographic conditions concluded Hypersil C18(4.6 mm×200 mm,5 m) column with the mobile phase consisting of acetonitrile-0.1% phosphoric acid solution in gradient elution mode at the flow rate of 0.9 mL·min-1.The detection wavelengths were 210,210,276,276 nm respectively.Results Catalpol,verbascoside,atractylenolide-I and atractylenolide-Ⅲ in Dangshen Yangrong pills had good linearity in the ranges of 0.0586～1.1720 μg(r=0.9997),0.0124～0.2480 μg(r=0.9992),0.0106～0.2120 μg(r=0.9994),0.0172～0.3440 μg(r=0.9996) respectively. The average recoveries of four constituents were 97.85％(RSD=1.13％),96.71％(RSD=0.77％),97.55％(RSD=1.22％) and 98.01％(RSD=1.04％) respectively. Conclusion This method is simple, accurate, repeatable and can be used for the quality control of Dangshen Yangrong pills.

Key words: Dangshen Yangrong pills, catalpol, verbascoside, atractylenolide-I, atractylenolide-Ⅲ