Abstract: Objective To develop a HPLC method for determination of indirubin in Daige powders. Methods The optimum HPLC conditions were as follows Hypersil C18 column(4.6 mm× 250 mm,5μm), methanol-water(7030)as mobile phase, detection wavelength 292nm, column temperature 30℃, flow rate 1mL·min-1. Results There was good linearity between peak areas and injection quantity of indirubin within the range of 0.01~0.20μg(r=0.9999).The average recovery was 98.5% for indirubin(RSD=1.00%). Conclusion The method can detect indirubin in Daige powders. The HPLC method is reasonable, stable and accurate.

Key words: Daige powders, indirubin, determination of content