Abstract: Objective To explore the protective effectsof Suhexiang pillsagainst septic myocardial injury induced by cecal ligation and puncture (CLP) in miceand dose-effect relationships in order to to provide data for interventionswith septic cardiomyopathyusing traditional Chinese medicine. Methods SPF male C57BL/6J mice were randomly divided into a sham operation group, model group, Suhexiang pill low dose group and high dose group (0.195, 0.39gdian·kg^-1). A sepsis myocardial injury model was established using the CLP method. Ligation and fixation were performed under the connecting rod of the ileocecal junction, and a penetrating puncture was made with the 21 G needle. After modeling, the rats were intragastrically administrated for 7 consecutive days. The condition and 7-day survival rate of these mice were observed, the cardiac function parameters of echocardiography were detected, and the pathomorphology of myocardia and lung tissues was observed via HE staining. Results Compared with the sham operation group, the mice in the model group were depressed, weight gain was slow, and the 7-day mortality was 70%. The lactic acid level increased significantly, the left ventricular ejection fraction and short axis shortening rate decreased significantly while the ventricular end systolic volume and left ventricular systolic diameter increased. The myocardial fibers became disordered and dissolved, and the infiltration of inflammatory cells in lung tissue was pronounced. Compared with the model group, the general stateof the two dose groups of Suhexiang pills was improved, the acid-base imbalancecorrected, lactic acid contents reduced, survival rateincreasedsignificantly, cardiac function indexes improved and histopathological damage mitigated. A high dose had significant advantages in improving survival rate, reducing lactic acid levels and lessening tissue damage. Conclusions uhexiang pills have a marked protective effect against CLP-induced septic myocardial injury in mice by improving the internal environment of the whole body, enhancing cardiac function, reducing tissue damage and lowering mortality. The efficacy is dose-dependent. High-dose interventionsare more effective.

Key words: Suhexiang Pills, Sepsis Myocardial Injury, Cecal Ligation and Perforation Model, Cardiac Function, Dose Effect, Mice