Research Progress in Applications of MAT

doi:10.19803/j.1672-8629.20250672

Abstract

Abstract: Objective To develop a more sensitive, broad-spectrum, three-R (replacement, reduction and refinement) and non-animal in vitro platform in order to address ethical concerns, species-specific variations, and limited ranges of detection inherent in conventional methods for detection of pyrogens. Methods By reviewing the related literature, the characteristics, research progress and developments concerning the monocyte activation test (MAT) were summarized. Results The MAT technology proved capable of sensitive and broad-spectrum detection of endotoxins and non-endotoxin pyrogens (NEPs) In addition, this technology could be used entirely in vitro and without using any animal. Conclusion This study has validated the MAT as a robust and ethically-feasible technology for ensuring the safety and efficacy of drugs.

Key words: Pyrogen, Endotoxin, Non-Endotoxin, Monocyte Activation Test, Pharmacopoeia

CLC Number:

R927.1

WU Hao, HE Qing, CAI Tong, PEI Yusheng, LIU Tao, WANG Dongmei. Research Progress in Applications of MAT[J]. Chinese Journal of Pharmacovigilance, 2026, 23(2): 213-218.

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https://zgywjj.magtechjournal.com/EN/Y2026/V23/I2/213

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