Spectrum-fect relationship of Ginseng Radix et Rhizoma Rubra and Polygonum Multiflorum Radix Preparata medicinal pair in protection of nerve cells

doi:10.19803/j.1672-8629.20230110

Abstract

Abstract: Objective To establish ultrahigh performance liquid chromatography (UHPLC) fingerprints of Ginseng Radix et Rhizoma Rubra(GR) and Polygonum Multiflorum Radix Preparata (PMRP) for different extraction processes, and to study the spectrum-effect relationship with the protective effect against Aβ_1-42 induced HT-22 cell injury. Methods 30% ethanol reflux extracts, 30% ethanol percolation extracts, 70% ethanol reflux extracts, 70% ethanol percolation extracts and water extracts from GR and PMRP were prepared. UHPLC fingerprints for different extraction processes were established using the UHPLC method combined with the Similarity Evaluation System of TCM Chromatogramtic Fingerprint (2012A) before the common peaks were identified. Aβ_1-42was used to induce HT22 cells of hippocampal neurons. The protective effect of extracts using different processes on the model was evaluated with the MTT method. Using the protective effect against Aβ_1-42induced HT-22 cell injury as indexes, the properties of extracts using different processes were investigated. The correlations between the common peaks of UHPLC fingerprints and the indexes of analgesic and protective effect were analyzed via grey correlation analysis and the partial least square（PLS）method. Results There were 19 common peaks for different extraction processes of GR and PMRP, and 13 components were identified by reference comparison. Grey correlation analysis found that the degree of correlation between the 14 common peaks and neural cell protection exceeded 0.6, suggesting good correlations. PLS analysis indicated that peaks 6, 7, 8, 9, 10, 12, 13, 14, 17 and 18 had a greater impact on the protection of nerve cells. Conclusion UHPLC fingerprints for different extraction processes of GR and PMRP are established and 19 common peak components are identified. Emodin-8-O-β-D-glucoside, emodin methyl ether-8-O-β-D-glucoside, emodin, emodin methyl ether, 20(S)-ginsenoside Rg2, ginsenoside Ro, ginsenoside Rd and peaks 14, 17 and 18 may be the effective substances of neuroprotection.

Key words: Ginseng Radix et Rhizoma Rubra and Polygonum Multiflorum Radix Preparata medicinal pair, neuroprotection, fingerprint, spectrum-effect relationship, Aβ_1-42, HT-22 cells, grey correlation analysis, PLS

CLC Number:

R917

LIU Jingjing, DAI Zhong, YANG Jianbo, WANG Ying, XU Beilei, LIU Yue, WANG Qi, MA Shuangcheng. Spectrum-fect relationship of Ginseng Radix et Rhizoma Rubra and Polygonum Multiflorum Radix Preparata medicinal pair in protection of nerve cells[J]. Chinese Journal of Pharmacovigilance, 2023, 20(6): 623-628.

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