Effects of Bibenzyl Compound (20C) from Gastrodia elata on MPTP/p-induced Mice and Mechanism

doi:10.19803/j.1672-8629.2020.11.02

Abstract

Abstract: Objective To study the pathological and biochemical effect of bibenzyl compound 20C on the mouse model of MPTP/p (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, MPTP/probenecid) and explore the mechanism. Methods A mouse model of MPTP/p was established. Mice received an intraperitoneal injection of MPTP and subcutaneous injection of probenecid. Pathological changes were examined by HE staining and immunohistochemistry while biochemical changes were detected by Western blot. Results Pathological and biochemical results showed that 20C could reduce expressions of MPTP/p-induced nitrated-α-synuclein in the stomach. The results of Western blot suggested that 20C reduced the level of Toll-like receptors 4 (TLR4) in the MPTP/p model, decreased phosphorylated nuclear factor-κB (NF-κB) p65 (p-p65), inhibited interleukin-1β (IL-1β) generation, and ameliorated inflammation. Conclusion 20C can reduce the formation of α-synuclein aggregation and nitrated-α-synuclein, inhibit TLR4-NF-κB p65 pathway, prevent p65 from aetivation, decrease IL-1β, inhibit inflammation and protect mice from MPTP toxicity.

Key words: Parkinson's disease, MPTP/p, bibenzyl compound 20C from Gastrodia elata, α-synuclein

CLC Number:

R965

ZHOU Tiantian, ZHANG Qiushuang, CHEN Ying, ZHU Chenggen, SHI Jiangong, CHEN Naihong, YUAN Yuhe. Effects of Bibenzyl Compound (20C) from Gastrodia elata on MPTP/p-induced Mice and Mechanism[J]. Chinese Journal of Pharmacovigilance, 2020, 17(11): 753-757.

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