中国药物警戒 ›› 2014, Vol. 11 ›› Issue (6): 321-324.

• 基础与临床研究 •    下一篇

附子不同组分单次给药对小鼠神经毒性的“时-毒”关系研究

王懿1, 2, 孙蓉1*, *   

  1. 1山东省中医药研究院,山东 济南 250014;
    2山东中医药大学,山东 济南 250355
  • 收稿日期:2014-05-21 修回日期:2016-02-03 出版日期:2014-06-08 发布日期:2016-03-02
  • 通讯作者: 孙蓉，博士，研究员·博导，中药药理与毒理。E-mail：sunrong107@163.com
  • 作者简介:王懿，女，在读硕士，中药药理与毒理。

Time-toxicity Relationship Study on Neurotoxicity Caused by Different Components of Radix Aconite Lateralis to Mice

WANG Yi1, 2, SUN Rong1, *   

  1. 1Shandong Research Academy of TCM, Shandong Jinan 250014, China;
    2Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China
  • Received:2014-05-21 Revised:2016-02-03 Online:2014-06-08 Published:2016-03-02

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摘要： 目的研究附子不同组分单次给药致小鼠神经毒性的时毒关系。方法取小鼠按不同时间点或不同组分分组,分别给小鼠灌胃附子不同组分,观察小鼠死亡情况和毒性反应,分别于药后相应时间点观察记录小鼠自主活动数和转棒数,检测血清过氧化物酶（SOD）、丙二醇（MDA）、乳酸脱氢酶（LDH）活性以及神经元特异性烯醇化酶（NSE）含量变化。结果水提组分组小鼠自主活动先加强后减弱再加强,血LDH药后60min升高显著;醇提组分组小鼠自主活动先减弱后加强,转棒停留时间先减少或增多,60min降低显著,血SOD含量在15~120min内持续降低;全组分组小鼠自主活动和转棒停留时间均在90min减少显著,血SOD含量减低,但显著性差异小于醇提组分。3种组分组小鼠血清的神经元特异性烯醇化酶（NSE）含量均有不同程度的升高,且醇提>水提>全组分。结论单次给小鼠灌胃一定剂量的附子不同组分可造成一定的神经损伤,且醇提组分的损伤程度最大,并呈现一定的神经毒性“时-毒”关系。对损伤机制和进一步损伤所导致的行为学、递质水平、神经元等留待后续研究。

关键词: 附子, 不同组分, 神经毒性, “, 时-毒”, 关系

Abstract: ObjectiveTo study the time-toxicity relationship caused by different components of Radix Aconite Lateralis to mice. MethodsMice were grouped according to different time, to observe the death condition and toxicity of mice. The numbers of autonomic activities and rotarod test were observed and recorded. The changes of the activity of SOD, MDA, LDH and NSE were detected. ResultsThe changes of autonomic activities of mice with water extraction were strengthened and then weakened, the changes of LDH in serum were lifted marked at 30 minutes. The changes of autonomic activities and standing time of mice with alcohol extraction were weakened and then strengthened, significant reduction was at 60 minutes, the changes of SOD in serum were reducing marked from 15 to 120 minutes. The changes of autonomic activities and standing time of mice with whole components were reduced at 90 minutes, the changes of SOD in serum were reducing. The levels of neuron specific enolization enzyme(NSE) from the three groups' mice serum were changed to rise in different degree, and alcohol extraction > water extraction > the whole components. ConclusionSingle intragastric administrations of different components of Radix Aconite Lateralis with certain dosage may induce acute nerve injury in mice and show certain time-toxicity relationship. The study on damage mechanism and the change of behavioral science, neurotransmitter levels and neuron would be discussed in the following study.

Key words: Radix Aconite Lateralis, different components, neurotoxicity, time-toxicity relationship

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