中国药物警戒 ›› 2014, Vol. 11 ›› Issue (2): 77-80.

• 益母草安全性研究 • 上一篇    下一篇

复方配伍对益母草急性毒性和总生物碱含量影响的实验研究

李晓宇1, 冯群2, 吕丽莉1, 孙蓉1*, *   

  1. 1山东省中医药研究院,山东 济南 250014;
    2天津中医药大学,天津 300073
  • 收稿日期:2013-01-31 修回日期:2016-02-03 出版日期:2014-02-08 发布日期:2016-02-03
  • 通讯作者: 孙蓉,女,研究员,博士生导师,中药药理与毒理研究。E-mailsunrong107@163.com
  • 作者简介:李晓宇,女,研究实习员,中药药理与毒理研究。
  • 基金资助:
    基金项目：国家中医药管理局中医药科学技术研究专项“有毒中药”质量控制及有毒成分限量示范研究（2004ZX00）

Experimental Research of Influences on Acute Toxicity and Content of Total Alkaloids in Herba Leonuri Caused by Combinations

LI Xiao-yu1, FENG Qun2, LV Li-li1, SUN Rong1, *   

  1. 1Shandong Academy of Chinese Medicine, Shandong Ji'nan 250014, China；
    2Tianjin University of Traditional Chinese Medicine, Tianjin 300073, China
  • Received:2013-01-31 Revised:2016-02-03 Online:2014-02-08 Published:2016-02-03

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摘要： 目的对益母草的总生物碱含量和急性毒性大小进行研究,探讨不同配伍对益母草的总生物碱类化学成分含量与急性毒性的影响。方法运用紫外分光光度法、经典急性毒性实验法,进行不同配伍方式中益母草总生物碱的含量测定及急性毒性比较研究。结果益母草、益母草+当归、益母草+川芎、益母草+木香和益母丸全方样品中,益母草总生物碱的含量依次为0.51%,0.34%,0.37%,0.37%,0.36%。益母草组无法做出LD50值,其MTD值为2.34 g·kg-1,相当于临床70 kg人每kg体重日用量的218.8倍,其中2只小鼠死亡;益母草+当归、益母草+川芎、益母草+木香和益母丸全方的MLD值相当于含益母草生药量分别为2.34,3.52,3.23,3.60,3.32 g·kg-1,分别相当于临床70 kg人每kg体重日用量的328.8,301.6,336.3,310.0倍,连续观察14 d,小鼠一般状况良好,无死亡。结论不同配伍对益母草中总生物碱的含量和毒性各不相同,其最大耐受量提示益母草毒性较小,可通过与当归、川芎、木香等中药配伍降低益母草中总生物碱的含量达到减毒的目的。

关键词: 益母草, 配伍, 总生物碱, 急性毒性

Abstract: ObjectiveTotal alkaloids contents and acute toxicity of Herba Leonuri were researched to probe the influence caused by different combinations. MethodsUV spectrophotometry was used to measure the content of total alkaloids and the classical method of acute toxicity was used to compare the difference of acute toxicity among different combinations. ResultsThe contents of total alkaloids in the samples of Herba Leonuri, Herba Leonuri + Angelica sinensis, Herba Leonuri+ Ligusticum chuanxiong, Herba Leonuri + Aucklandia lappa and the whole formula were separately 0.51%, 0.34%, 0.37%, 0.37%, 0.36%. Calculated by crude drug content of Herba Leonuri, the MTD of Herba Leonuri was 2.34 g·kg-1 and the MLD of other for samples were respectively 3.52, 3.23, 3.60, 3.32 g·kg-1, which were respectively equal to 218.8, 328.8, 301.6, 336.3, 310.0 times of 70 kg person's daily dried medicinal herb expenses. Making continuous observation of 14 days, mice were generally in good condition and only two mice in the group of Herba Leonuri were dead. ConclusionThe content of total alkaloids and the acute toxicity of Herba Leonuri are closely related to compatibilities. The MTD shows the basic security and low toxicity of Herba Leonuri. The combination of Angelica sinensis, Ligusticum chuanxiong or Aucklandia lappa with Herba Leonuri can reduce toxicity via reducing the content of total alkaloids in Herba Leonuri.

Key words: Herba Leonuri, compatibility, total alkaloids, acute toxicity

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