中国药物警戒 ›› 2014, Vol. 11 ›› Issue (11): 661-663.

• 质量与工艺研究 • 上一篇    下一篇

高效液相色谱法测定复方生地合剂中绿原酸的含量

许伟英, 郑新颖, 谷晓颖, 任世禾   

  1. 上海中医药大学附属市中医医院，上海 200071
  • 收稿日期:2014-06-17 修回日期:2018-07-18 出版日期:2014-11-08 发布日期:2016-03-02
  • 通讯作者: 任世禾，女，主任药师，教授，医院药学。E-mail:0190@szy.sh.cn
  • 作者简介:许伟英，女，主管药师，硕士，药品不良反应监测。
  • 基金资助:
    上海市科委中药现代化专项（11DZ1972301）。

Determination of Chlorogenic Acid in Fufang Shengdi Mixture by HPLC

XU Wei-ying, ZHENG Xin-ying, GU Xiao-ying, REN Shi-he   

  1. Shanghai Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai TCM University, Shanghai 200071, China
  • Received:2014-06-17 Revised:2018-07-18 Online:2014-11-08 Published:2016-03-02

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摘要： 目的 建立复方生地合剂中绿原酸含量测定的高效液相色谱法。方法 色谱柱（250 mm×4.6 mm，5μm），流动相：乙腈-0.4%磷酸溶液（10：90），流速1 mL·min-1，柱温30℃，检测波长327 nm。结果 绿原酸在0.0624~4.68μg范围内线性关系良好。R=0.9999，平均回收率为100.7%，RSD=1.7%（n=6）。结论 该方法操作简便，重复性高，可用于复方生地合剂的质量控制。

关键词: 高效液相色谱法（HPLC）, 复方生地合剂, 绿原酸

Abstract: Objective To establish a HPLC method for the determination of chlorogenic acid in Fufang Shengdi mixture. Methods A SHISEIDO MG C18 column(250 mm×4.6 mm, 5μm) was used with the mobile phase of 0.4% phosphoric acid and acetonitrile for isocratic elution (10:90) at the wavelength of 327 nm, with the flow rate of 1mL·min-1 and the column temperature was 30℃. Results The calibration curves for chlorogenic acid were linear in the concentration ranges of 0.0624～4.68 μg, and the correlation coefficient was 0.999999. The average recovery was 100.7%,(RSD=1.7%,n=6). Conclusion This method is simple and reproducible,and is suitable for the quality control of the Fufang Shengdi mixture.

Key words: high pressure liquid chromatography(HPLC), Fufang Shengdi mixture, chlorogenic acid

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