中国药物警戒 ›› 2014, Vol. 11 ›› Issue (11): 641-644.

• 基础与临床研究 •    下一篇

基于物质基础和抗脑缺血作用的芎汤加味方提取工艺研究

冯群1,2, 迟雪洁3, 张亚囡4, 孙蓉1   

  1. 1 山东省中医药研究院，山东 济南250014;
    2 天津中医药大学，天津300073;
    3 潍坊市中医院，山东 潍坊261041;
    4 济宁市药品检验所，山东 济宁272000
  • 收稿日期:2014-07-30 修回日期:2018-07-18 出版日期:2014-11-08 发布日期:2016-03-02
  • 通讯作者: 孙蓉，女，研究员·博导，中药药理与毒理研究。E-mail：sunrong107@163.com
  • 作者简介:冯群，男，在读硕士，中药药理与毒理研究。
  • 基金资助:
    重大新药创制专项：中药复方药理学研究及药效学评价关键技术（2009ZX09502-015）。

Research of Extraction Process of Modified Xiongqiong Decoction Based on Material Basis and Effect of Anti-cerebral Ischemia

FENG Qun1,2, CHI Xue-jie3, ZHANG Ya-nan4, SUN Rong1   

  1. 1 Shandong academy of Chinese Medicine, Shandong Ji'nan 250014, China;
    2 Tianjin University of Traditional Chinese Medicine, Tianjin 300073, China;
    3 Weifang Traditional Chinese Hospital, Shandong Weifang 261041, China;
    4 Jining Institute for Drug Control, Shandong Jining 272000, China
  • Received:2014-07-30 Revised:2018-07-18 Online:2014-11-08 Published:2016-03-02

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摘要： 目的 在小鼠全脑缺血再灌注损伤模型的基础上，根据芎汤加味方所含物质基础研究进展，进行其提取工艺研究。方法 结合文献进行芎汤加味方6个制备工艺的研究，测定各工艺提取物中有效成分的含量，并观察各个提取物对小鼠全脑缺血再灌注损伤模型的保护作用。通过物质基础的含量测定和抗脑血活性的比较，筛选最有效的提取工艺。结果 通过挥发油、核桃油、阿魏酸提取率指标进行综合评价，工艺5提取物的有效成分保留相对全面，对小鼠全脑缺血和缺氧损伤程度的改善作用最强。结论 采用“质量-药效”综合评价模式可保障中药复方的有效性。初步确定了工艺5为芎汤加味方的最佳提取工艺，其具体优化参数有待于进一步研究。

关键词: 芎 汤加味方, 全脑缺血再灌注损伤, 提取工艺, 含量测定

Abstract: Objective Extraction process of modified Xiongqiong decoction was researched and optimized on the basis of mice models of cerebral ischemia-reperfusion damages according to its material basis. Methods Six extraction processes of modified Xiongqiong decoction were explored according to modern references. Contents of active ingredients in the extracts were determined and protective effects of them on mice models of cerebral ischemia-reperfusion damages were observed. Optimum extraction process was selected according to determination of material basis and comparison of anti-cerebral ischemia. Results Active ingredients in the extract of the fifth extraction process is comprehensive relatively according to the integrated evaluation to volatile oil, walnut oil and ferulic acid. Extract of the fifth extraction process improved most obviously cerebral ischemia-reperfusion damages in all the extracts. Conclusion Model of "quality-efficacy" comprehensive assessment can guarantee effectiveness of Chinese medicine compound. The fifth extraction process has been confirmed to be optimum extraction process of modified Xiongqiong decoction preliminarily. However specific optimum parameters of the fifth extraction process need to be confirmed by a further study.

Key words: modified Xiongqiong decoction, cerebral ischemia-reperfusion damages, extraction process, determination

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