中国药物警戒 ›› 2015, Vol. 12 ›› Issue (4): 203-206.

• 柴胡药材、有效成分及制剂的“毒-效”及质量评价专栏 • 上一篇    下一篇

柴胡水提组分对大鼠解热及伴随毒副作用实验研究

石亮1，2,黄娜娜2,孙晓倩2,李晓宇1,孙蓉1*   

  1. 1山东省中医药研究院，山东 济南 250014;
    2山东中医药大学，山东 济南 250355
  • 收稿日期:2015-03-31 出版日期:2015-04-08 发布日期:2015-07-27
  • 通讯作者: 孙蓉，女，博士，研究员?博导，中药药理与毒理。
  • 作者简介:石亮，男，在读硕士，中药药理与毒理。
  • 基金资助:
    国家自然科学基金面上项目（81374059），山东省自主创新和成果转化项目（2014ZZCX02104）, 山东省自然基金重点项目（2011ZRB14333）。

Study on Rat's Antipyretic Effect Accompanied by Side Effects of Water Extraction Components of Bupleurum

SHI Liang1,2,HUANG Na-na2,SUN Xiao-qian2,LI Xiao-yu1,SUN Rong1*   

  1. 1Shandong Academy of Chinese Medicine, Shandong Jinan 250014, China;
    2Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China
  • Received:2015-03-31 Online:2015-04-08 Published:2015-07-27

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摘要： 目的 研究柴胡水提组分对大鼠发热模型的解热作用及伴随的毒副作用，为其“功效-毒性”相关性研究提供实验依据。方法 采用背部皮下注射2，4-二硝基苯酚，制备大鼠发热模型，灌胃不同剂量柴胡水提组分，每30 min测体温1次，连续测定4 h，记录肛温，观察其解热作用；检测血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、总胆汁酸（TBA）、总胆红素（TBIL)、肌酐（Cr）、尿素氮（BUN）的活性及含量，剖取心脏、肝脏、肾脏称重，计算心、肝、肾各脏体比值，评价其药效伴随毒副作用。结果 柴胡水提组分对2, 4-二硝基苯酚所致大鼠发热模型有降温作用，给药3 d后血清ALT、AST活性明显升高，肝体比值无明显变化。结论 药效剂量的柴胡水提组分对大鼠发热模型有一定的降温作用，同时会产生一定的肝毒性，并呈现一定的剂量依赖关系。

关键词: 柴胡, 解热, 伴随毒副作用

Abstract: Objective To study the antipyretic effects and accompanied side effects of water extraction of Bupleurum components for rat fever model so as to provide the experimental basis for "efficacy- toxicity" correlation. Methods Rat fever model was established via the back injection of 2,4-dinitrophenol (DNP). Different doses of water extraction of Bupleurum components were given to rats by gavage. The temperature was detected in anus once every 30 minutes, continuously determined for 4 hours, recorded anal temperature and observed the antipyretic effect. At the same time, the levels of ALT, AST, TBA, TBIL, Cr and BUN in serum were detected and the heart, liver and kidney indexes were calculated. Efficacy and accompanied side effects were evaluated via all these data. Results Antipyretic effect was significantly reflected when rat fever model caused by DNP were treated with water extraction of Bupleurum components by gavage. The activities of ALT and AST increased obviously after administration for consecutive 3 days. Conclusion Water extraction of Bupleurum components has the effect of dropping in temperature to the rat fever model, which is accompanied by hepatotoxicity. The effect and hepatotoxicity all show a certain dose dependence relationships.

Key words: Bupleurum, antipyretic effect, accompanied side effect

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