中国药物警戒 ›› 2012, Vol. 9 ›› Issue (5): 269-271.

• 有毒中药“质量-毒性”关联评价 • 上一篇    下一篇

提取方式对鸦胆子“质量-毒性”综合评价模式的影响

朱兰兰1，2，孙蓉2，*   

  1. 1 天津中医药大学，天津 300193；
    2 山东中医药研究院，山东 济南 250014
  • 收稿日期:2012-03-29 出版日期:2012-05-10 发布日期:2015-08-10
  • 通讯作者: 蓉,女,博士生导师,研究员,中药药理、毒理与新药研究。E-mail：sunrong107@163.com
  • 作者简介:朱兰兰，女，硕士研究生，药物分析与中药毒代研究。
  • 基金资助:
    国家重点基础研究发展计划 （973）中医基础理论专项资助项目（2009CB522802）

The Effect of Extracting Methods on "Quality Toxicity" Comprehensive Evaluation Model of Fructus Bruce.

ZHU Lan-lan1, 2, SUN Rong2, *   

  1. 1 Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;
    2 Shandong Academy of Chinese Medicine, Shandong Jinan 250014, China
  • Received:2012-03-29 Online:2012-05-10 Published:2015-08-10

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摘要： 目的对不同提取方式提取的鸦胆子中油酸含量和急性毒性大小进行比较研究，探讨不同提取方式对鸦胆子“含量-毒性”综合评价模式的影响。方法用GC法测定鸦胆子水提组分、醇提组分中油酸的含量，并进行方法学考察;经典急性毒性实验法，进行鸦胆子不同提取物的急性毒性比较研究。结果鸦胆子水提物、醇提物中油酸的百分含量分别为1.83%、10.35%;鸦胆子水提物、醇提物的小鼠口服LD50分别为4.023g·kg-1、3.320g·kg-1，腹泻、尾部紫绀是主要的急性毒性症状。结论提取方式不同，鸦胆子中油酸的含量和毒性各不相同，其LD50显示鸦胆子经不同方式的提取均具有毒性，其提取物对小鼠急性毒性强度为：醇提物组> 水提物组;提取物中油酸的含量依次为：醇提物组> 水提物组。说明提取方式对吴茱萸“质量-毒性”综合评价模式有一定的影响，其毒性大小可能与主要化学成分含量存在一定相关性。因此通过“质量-毒性”综合评价模式确定有毒中药的提取方式，更全面客观合理。

关键词: 鸦胆子, 提取方式, 含量, 急性毒性, 综合评价

Abstract: Objective Explore the effect of extracting methods on "quality toxicity" comprehensive evaluation model by comparing the content of alkaloid and acute toxicity from Brucea javanica(L.)Merr. extracted by different methods. MethodsGC was used to determine the content of oleic acid in water extract and alcohol extract of Brucea javanica(L.)Merr.; the classical method of acute toxicity was used to determine the acute toxicity of Brucea javanica(L.)Merr. at different extraction, and compare the acute toxicity. ResultsThe content of oleic acid which included water extract and ethanol extract was separately1.83%、10.35%. The LD50 of water extract and alcohol extract of Fructus Brucea to ice were 4.023g·kg-1, 3.320g·kg-1. Diarrhea and cyanosis are main acute toxic symptoms. ConclusionThe content of oleic acid and acute toxicity of the Fructus Brucea by different extracting methods were different, and death occurred in each sample, the LD50 showed that the different extracts which extracted from the Fructus Brucea at different extracting methods had certainly toxicity in mice. The acute toxicity of different extracts on mice is: alcohol extraction group>water extraction group, the content of oleic acid in different extracts is: alcohol extraction group>water extraction group. Suggesting that it was certain effect of extracting methods on "quality toxicity" comprehensive evaluation model. It was more comprehensive, Objective and reasonable to determine extraction methods of toxic Chinese medicine through the comprehensive evaluation model.

Key words: Fructus Bruce, evaluation model, quality, toxicity, comprehensive evaluation