中国药物警戒 ›› 2015, Vol. 12 ›› Issue (6): 338-340.

• 质量与工艺研究 • 上一篇    下一篇

HPLC法同时测定七味清咽气雾剂中苦参碱、射干苷和次野鸢尾黄素的含量

孟蕾蕾   

  1. 日照市食品药品检验检测中心，山东日照 276800
  • 收稿日期:2015-02-28 出版日期:2015-06-08 发布日期:2015-07-27
  • 作者简介:孟蕾蕾，女，本科，主管药师，药品检验。

Determination of Martine, Belamcandin and Irisflorentin in Qiweiqingyan Aerosol by HPLC

MENG Lei-lei   

  1. Rizhao Center for Food and Drug Control/Testing, Shangdong Rizhao 276800, China
  • Received:2015-02-28 Online:2015-06-08 Published:2015-07-27

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摘要： 目的 建立HPLC法测定七味清咽气雾剂中苦参碱、射干苷和次野鸢尾黄素的含量。方法 色谱柱为Agilent Zorbas Stablebond C18柱（4.6 mm×250 mm，5μm）；流动相组成为乙腈（A）-甲醇（B）-0.1％磷酸水溶液（C）,梯度洗脱,检测波长为251nm，柱温为30℃，流速为1mL?min-1。结果 苦参碱、射干苷和次野鸢尾黄素分别在0.25～125 μg?mL-1，0.10～101μg?mL-1，0.12～234μg?mL-1范围呈良好的线性关系，与峰面积积分值的线性关系良好，平均回收率为98.5 %～100.6%之间。结论 本方法 可用于测定七味清咽气雾剂中苦参碱、射干苷和次野鸢尾黄素的含量，结果 准确，重复性好。

关键词: 七味清咽气雾剂, 苦参碱, 射干苷, 次野鸢尾黄素, HPLC, 含量测定

Abstract: Objective To develop a HPLC method for determination of martine, belamcandin and irisflorentin in Qiweiqingyan aerosol. Method The optimum HPLC condition was as follows: Agilent Zorbas Stablebond C18 column(4.6 mm×250 mm,5 μm) with gradient elution of acetonituile (A)- methanol(B)-0.1% phosphoric acid (C), detection wavelength:251 nm, column temperature: 30℃, flow rate:1mL?min-1. Results There were good linearity between peak areas and injection quantity of martine, belamcandin and irisflorentin within the range of 0.25～125 μg?mL-1, 0.10～101μg?mL-1, 0.12～234μg?mL-1.The average recoveries were between 98.5 %～100.6%. Conclusion The method could detect martine, belamcandin and irisflorentin in Qiweiqingyan aerosol. The HPLC measurement was reasonable, stable and accurate.

Key words: Qiweiqingyan aerosol, martine, belamcandin, irisflorentin;HPLC, determination of content

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