中国药物警戒 ›› 2011, Vol. 8 ›› Issue (7): 388-390.

• 基础及临床研究 • 上一篇    下一篇

褪黑素对阿霉素致心肌损伤保护作用的实验研究

孙云鹏1, 张宇2, 李天书2, 温得中2, 章宏2, *   

  1. 1.吉林大学第一医院心外科,吉林长春130021;
    2.吉林大学白求恩医学院,吉林 长春130021
  • 收稿日期:2011-04-19 出版日期:2011-07-10 发布日期:2015-07-13
  • 通讯作者: *通讯作者：章宏,男,博士,心血管生理研究。E-mail：hzhang@jlu.edu.cn
  • 作者简介:孙云鹏,男,医学硕士,循环生理学。
  • 基金资助:
    吉林省卫生厅科研课题资助项目（基金编号：2009ZC048）

Protective Effect of Melatonin on Adriamycin-induced Myocardial Injury in Rats

SUN Yun-peng1, ZHANG-Yu2, LI Tian-Shu2, WEN De-zhong2, ZHANG Hong2, *   

  1. 1.Department of Cardiovascular Surgery, First Hospital, Jilin University, Jilin Changchun 130021, China;
    2.Norman Bethune College of Medicine Jilin University, Changchun 130021, China
  • Received:2011-04-19 Online:2011-07-10 Published:2015-07-13

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摘要： 目的 观察褪黑素（Melatonin,MLT）对阿霉素（Adriamycin）诱导的原代乳鼠心肌细胞的保护作用及其量效关系。方法 采用体外培养乳鼠心肌细胞,用阿霉素制备心肌细胞损伤模型,实验设正常对照组（C组）、阿霉素组（在培养液中加入阿霉素lmg/L）及在加入阿霉素前30分钟给予褪黑素（MLT）50、100、200 mol·L-1组（M1,M2,M3组）。观察褪黑素对体外培养的各组乳鼠心肌细胞脂质过氧化物丙二醛（MDA）及MTT代谢率的影响。结果 与阿霉素心肌细胞损伤组比较,褪黑素保护组的MDA释放量显著减少（P <0.01）,而MTT代谢率显著增高（P<0.01）,作用具剂量依赖性。结论 MLT对阿霉素诱导心肌细胞损伤有保护作用,并呈一定的剂量依赖性关系。

关键词: 褪黑素, 阿霉素, 心肌细胞凋亡, 心肌保护

Abstract: Objective To observe the Melatonin(MLT) on Adriamycin induced neonatal rat cardiomyocytes in primary protective effect of dose-effect relationship. Methods Cultured neonatal rat cardiomyocytes with Adriamycin Preparation of cardiac cell injury model, experimental and normal control group(C group), Adriamycin Group(ADM in the culture medium Jiaru A lmg/L) and 30 minutes before joining the Adriamycin given MLT 50,100,200 μmol·L-1 group(M1, M2, M3 group). Observation of melatonin on cultured neonatal rat cardiomyocytes in each group malondialdehyde(MDA) and MTT metabolic rate. Results Myocardial cell injury and Adriamycin group, protection group, MDA release melatonin significantly reduced(P <0.01), and MTT metabolic rate was significantly higher(P <0.01), with a dose-dependent effect. Conclusion MLT on Adriamycin induced a protective effect of myocardial cell injury and a dose-dependent relationship.

Key words: melatonin, Adriamycin, cardiomyocyte apoptosis, myocardial protection

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