中国药物警戒 ›› 2016, Vol. 13 ›› Issue (8): 449-451.

• 基础与临床研究 •    下一篇

三黄泻心汤“炮制配伍”解热优势方药效及其作用机制研究

马晓静1, 王岚1, 殷小杰1, 李丽1, 刘颖1, 贡磊磊1, 原会俊2, 肖永庆1, 梁日欣1*, *   

  1. 1 中国中医科学院中药研究所,北京 100700;
    2 首都医科大学中医药学院,北京 100069
  • 收稿日期:2016-09-14 修回日期:2016-09-14 出版日期:2016-08-20 发布日期:2016-09-14
  • 通讯作者: 梁日欣,女,博士·研究员,博导,中药药理及药代动力学研究。E-mail：liangrixin2009@sina.com
  • 基金资助:
    国家自然基金面上项目（81274087）

Study on Efficacy and Mechanisms of Antipyretic of Sanhuangxiexin Decoction by Processing Compatibility

MA Xiao-jing1, WANG Lan1, YIN, LI Li1, LIU Ying1, GONG Lei-lei, XIAO Yong-qing1, LIANG Ri-xin1, *   

  1. 1 Institute of Chinese Materia Medica, China Academy of Chinese Medicine Sciences, Beijing 100700, China;
    2 School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
  • Received:2016-09-14 Revised:2016-09-14 Online:2016-08-20 Published:2016-09-14

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摘要： 目的 观察三黄泻心汤“炮制配伍”解热优势方药效并初步探究其作用机制。方法 大鼠皮下注射20%干酵母混悬液复制发热模型,阿司匹林（0.1g·kg-1）作为阳性对照药,三黄泻心汤解热优势方分为3个剂量（1、0.5、0.25 g生药·kg-1）,灌胃给药。首先测量给药前基础体温,然后分别于给药后0.5、1、2、4 h测量大鼠肛温,以实测体温-基础体温（ΔT）表示体温变化,实验结束后采血及分离血清,取下丘脑组织后制备上清。Elisa法测定血清和下丘脑组织中PGE2、cAMP、Cox-1、Cox-2的含量。结果 三黄泻心汤“炮制配伍”解热优势方各剂量组均可使体温下降,并降低发热大鼠血清和下丘脑组织中PGE2、cAMP、Cox-1、Cox-2的含量。结论 三黄泻心汤“炮制配伍”解热优势方有明显的解热作用,其机制可能是与抑制PGE2、cAMP等中枢发热介质的释放有关。

关键词: 炮制配伍, 三黄泻心汤, 解热, 机制

Abstract: Objective To observe the antipyretic efficacy of Sanhuangxiexin decoction by processing compatibility and explore the mechanisms preliminarily. Methods The fever models were established by subcutaneous injection with dried yeast suspension. Aspirin was used as the positive control (0.1 g•kg-1), and Sanhuangxiexin decoction was divided into three doses （1, 0.5, 0.25 g crude drug•kg-1), which was intragastrically administrated. Firstly the basal body temperature was measured before administration, then a measurement of the fever temperature was carried out after 0.5, 1, 2, 4 h, which was expressed as ΔT. The blood was sampled to separate and collecte the serum, and the hypothalamus tissues were taken to prepare supernatant from rats after the experiment. Finally, Elisa method was used to test the content of PGE2, cAMP, Cox-1, Cox-2 both in the serum and hypothalamus. Results All three doses of Sanhuangxiexin decoction could decrease the temperature and the content of PGE2, cAMP, Cox-1, Cox-2 in serum and hypothalamus. Conclusion The formula 8 of Sanhuangxiexin decoction has obvious antipyretic effect, and its mechanisms maybe relate to inhibition of the release of centric febrile mediators such as PGE2 and cAMP, etc.

Key words: processing compatibility, Sanhuangxiexin decoction, antipyretic, mechanism

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