中国药物警戒 ›› 2010, Vol. 7 ›› Issue (9): 530-532.

• 柴胡毒性物质基础研究 • 上一篇    下一篇

柴胡总皂苷醇洗脱精制品对大鼠急性毒性实验研究

黄伟1,孙蓉2   

  1. 1.山东中医药大学,山东济南 250355；
    2.山东省中医药研究院,山东济南 250014
  • 收稿日期:2010-05-06 修回日期:2016-03-09 出版日期:2010-09-08 发布日期:2016-03-09
  • 通讯作者: 孙蓉,女,研究员,硕士生导师,中药药理与毒理。E-mail：sunrong107@163.com
  • 作者简介:黄伟,,男,在读硕士研究生,中药药理与毒理。
  • 基金资助:
    国家自然科学基金项目（30672649）；山东省科技平台建设项目（2008GG2NS02021）；山东省科技公关关键技术研究课题（2007GG2NS02073）；山东省国际合作引智项目（L20083700336）；国家863 科技攻关计划课题（2004BA721A15）；国家重点基础研究发展计划（973）中医基础理论专项资助项目（项目编号：2009CB522802）

Experimental Study on Rat's Acute Toxicity of Refined Products of Bupleurum Saikosaponin by Alcohol Elution

HUANG Wei1 SUN Rong2*   

  1. 1.Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China;2Shandong Research Academy of TCM, Shandong Jinan 250014, China
  • Received:2010-05-06 Revised:2016-03-09 Online:2010-09-08 Published:2016-03-09

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摘要： 目的 探讨柴胡总皂苷醇洗脱精制品对大鼠急性毒性的影响。方法 采用经典的急性毒性试验方法,进行柴胡总皂苷醇洗脱精制品对大鼠的急性毒性实验研究。结果 按含柴胡总皂苷含量计算,柴胡总皂苷醇洗脱精制品对小鼠的半数致死量（LD50）为591.95 mg·kg-1·d-1,95%可信限为490.96～713.7 mg·kg-1·d-1。死亡大鼠急性毒性主要表现为安静怠动,即而出现步态不稳、腹卧昏睡、心率加快、呼吸急促、连续性抽搐、神经抑制而死亡。存活大鼠在14 天观察期内体重增长缓慢,余未见明显异常。柴胡总皂苷醇洗脱精制样品的大鼠急性毒性比柴胡总皂苷粗提取物的急性毒性大,但比小鼠急性毒性小。结论 柴胡总皂苷是柴胡产生毒性的主要物质基础,其产生毒性的部位及毒性机制有待于深入研究。

关键词: 柴胡总皂苷, 分离精制, 大鼠, 急性毒性

Abstract: Objective To discuss the effect of refined products of Bupleurum saikosaponin by alcohol elution on rat's acute toxicity. Methods Make the study on rat's acute toxicity of the refined products of Bupleurum saikosaponin by alcohol elution in accordance with classical acute toxicity test methods. Results Caculated by total saikosaponin the LD50 of the refined products of Bupleurum saikosaponin by alcohol elution is 591.95 mg·kg-1·d-1, and the 95%(,) confidence limit is 490.96～713.7 mg·kg-1·d-1. The main acute toxicity symptoms and signs of dead rats are negligent action,then arising from unsteady gait, abdominal lying sleeping, rapid heart rate, shortness of breath, ontinuous convulsions, neurological inhibition, and eventually to death. The surviving rats' weight gainings are slow in 14d obervation period and there are no other obvious abnormalities. The acute toxicity of refined products of Bupleurum saikosaponin by alcohol elution is bigger than the total Bupleurum saikosaponin extracts,but it's smaller than the mice's. Conclusion The total saikosaponin is mainly toxical material basis of Bupleurum . The toxical location and mechanismare to be further studied.

Key words: total , separation and purification, rats, acute toxicity

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