中国药物警戒 ›› 2010, Vol. 7 ›› Issue (9): 513-514.

• 基础研究 •    下一篇

HPLC法测定刺五加果实中异嗪皮啶的含量

张海丰,滕坤,朱俊义   

  1. 通化师范学院,吉林通化 134000
  • 收稿日期:2010-07-20 修回日期:2016-03-09 出版日期:2010-09-08 发布日期:2016-03-09
  • 通讯作者: 朱俊义,男,教授,药用植物的功能饮品开发。E-mail: swx0527@163.com
  • 作者简介:张海丰,男,博士,中药化学与新药基础研究。

Determination of Isofraxidin in Medofenoxate Fruits by HPLC

ZHANG Hai-feng ,TENG Kun ,ZHU Jun-yi   

  1. Tonghua Normal University, Jilin Tonghua, 134000, China
  • Received:2010-07-20 Revised:2016-03-09 Online:2010-09-08 Published:2016-03-09

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摘要： 目的 建立HPLC测定刺五加果实中异嗪皮啶含量的方法。方法采用AgilentXDB-C18色谱柱（4.6mm×150mm,5 m）,以甲醇-0.2 %磷酸溶液（27 73）为流动相,流速为1.0mL·min-1,柱温为室温,进样量10 L,检测波长为343nm。结果异嗪皮啶进样量在0.334～4.175 g·mL-1内呈良好的线性关系,r=0.9996,平均加样回收率为100.74%,RSD 为0.44%（n=6）。结论该方法灵敏快速、结果准确、重现性好,可用于刺五加果实中异嗪皮啶含量的测定。

关键词: 刺五加果实, 异嗪皮啶, 高效液相色谱

Abstract: Objective To establisha HPLC method for the determination of isofraxidin in medofenoxate fruits. Methods Agilent XDB C18(4.6 mm伊150 mm,5μm) column was applied, using methanol-0.2% phosphoric acid (27：73)as mobile phase with the rate of 1.0mL·min-1. The column temperature was room temperature, the injection volumn was 10μL and the detective wavelength was 343 nm. Results A good linearity was obtained over the range of 0.835～4.176μg·mL-1 for isofraxidin(r=0.9996). The average recovery(n=6) was 100.74%(RSD=0.44%). Conclusion The method is practical, convenient, accurate, and can be used as the determination of isofraxidin in medofenoxate fruits.

Key words: medofenoxate fruit, isofraxidin, HPLC

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