中国药物警戒 ›› 2013, Vol. 10 ›› Issue (3): 133-135.

• 基础及临床研究 • 上一篇    下一篇

人参皂苷Rd对中国仓鼠肺细胞染色体畸变作用

高梅,曹冲,朱春花,曲保恩   

  1. 山东省医药工业研究所,山东省化学药物重点实验室,山东济南 250101
  • 收稿日期:2012-05-29 修回日期:2016-03-09 出版日期:2013-03-08 发布日期:2016-03-09

Effect of Ginsenoside Rd on Chromosome Aberration in Chinese Hamster Lung Cells

GAO Mei ,CAO Chong, ZHU Chun-hua, QU Bao-en   

  1. Shangdong Institute of Pharmaceutical Industry, Shandong Provincial Key Laboratory of Chemical Drug, Shandong Jinan 250101, China
  • Received:2012-05-29 Revised:2016-03-09 Online:2013-03-08 Published:2016-03-09

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摘要： 目的 研究人参皂苷Rd 致中国仓鼠肺细胞（Chinese hamster lung cells,CHL）染色体畸变的作用。方法 细胞计数法测定人参皂苷Rd 对CHL 细胞的半数抑制浓度（IC50）,根据IC50设立不同剂量组,进行染色体畸变试验,分别观察人参皂苷Rd 染毒6、24h 及加S9后染毒6h CHL 细胞染色体的数目及结构变化,进行染色体畸变分析。结果 人参皂苷Rd 染毒6、24h 及加S9后染毒6h CHL 细胞染色体畸变为阴性。结论 在本试验条件下,人参皂苷Rd 不能引起CHL 细胞染色体产生畸变。

关键词: 人参皂苷 Rd, 中国仓鼠肺细胞, 染色体畸变

Abstract: Objective To explore the effect of ginsenoside Rd on chromosome aberration in Chinese hamster lung cells(CHL). Methods We used the method of cell counting to determine the IC50 of ginsenoside Rd on CHL cells, then to establish the range of doses according to the IC50 and to do the cell chromosome aberration experiment. When the CHL cells were exposured to ginsenoside Rd at 6h and 24h and plusedS9 mixture at 6h respectively, we observed the changes of chromosome number and structure, then to judge the chromosome aberration Results . Results Negative response was found at 6h and 24h after the treatment with ginsenoside Rd and at 6h after the addition of S9 mixture. Conclusion Under the condition of this experiment, ginsenoside Rd does not induce chromosome aberration in CHL cells.

Key words: ginsenoside Rd, Chinese hamster lung cells(CHL), chromosome aberration

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