中国药物警戒 ›› 2013, Vol. 10 ›› Issue (4): 196-198.

• 基础及临床研究 • 上一篇    下一篇

糖尿病大鼠心肌组织AngⅡ变化及益气养阴散结中药复方对其影响的实验研究

杨晓晖,吴淑馨1,张力2,孙宏峰1   

  1. 1.北京中医药大学东方医院内分泌科,北京100078；
    2.国家食品药品监督管理局药品评价中心,北京100045
  • 收稿日期:2013-02-27 修回日期:2016-03-09 出版日期:2013-04-08 发布日期:2016-03-09
  • 作者简介:杨晓晖,男,主任医师,博士研究生导师,内分泌疾病的临床和基础研究。
  • 基金资助:
    北京中医药大学自主创新中青年教师资助项目（2009JYBZZ-1材料与方法JS103）

Effects of Yi-qi Yang-yin San-jie Herbal Compound on Cardiomyopathy of Diabetic Rats and the Expression of AngiotensinⅡ

YANG Xiao -hui1 ,WU Shu -xin1 ,ZHANG Li2 ,SUN Hong -feng1   

  1. 1..Endocrinology Department, Dongfang Affliated Hospital of Beijing University of Chinese Medicine, Beijing,100078, China;
    2..Center for Drug Reevaluation, SFDA, Beijing 100045, China
  • Received:2013-02-27 Revised:2016-03-09 Online:2013-04-08 Published:2016-03-09

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摘要： 目的 探讨益气养阴散结中药复方对糖尿病大鼠心肌组织AngⅡ蛋白表达的影响。方法 一次性腹腔注射链脲菌素（STZ）制造糖尿病大鼠模型,随机分为3 组：正常对照组、模型组及中药组,分别给药,中药组灌服中药,模型组及空白组灌服等量蒸馏水,4 周及8 周末分别处死大鼠,取出心脏固定包埋切片,采用HE 染色观察心肌组织的病理改变,免疫组化法检测心肌组织血管紧张素Ⅱ（AngⅡ）的表达,原位杂交检测AngⅡ mRNA 的表达,经图像分析后统计其光密度值的大小。结果 糖尿病病程4周及8周后,糖尿病模型组大鼠心肌组织AngⅡ含量以及AngⅡ mRNA 较正常组大鼠表达明显增高（ P<0.01）。而中药可以显著降低糖尿病模型大鼠心肌组织AngⅡ含量以及AngⅡmRNA表达（P <0.01）。结论 益气养阴散结中药复方可抑制糖尿病大鼠心肌细胞浆中AngⅡ及AngⅡ mRNA 的表达,减轻糖尿病心肌病变,其作用机制与抑制心脏局部肾素血管紧张素系统（RAS）激活有关。

关键词: 益气养阴散结, 糖尿病, 心肌病变, 肾素-血管紧张素系统

Abstract: Objective To study the effects of Yi-qi Yang-yin San-jie herbal compound on cardiomyopathy of diabetic rats and the expression of AngⅡ of myocardial tissue. Methods The diabetic model was established by streptozotocin injection to the abdominal cavities of rats.The male SD rats were randomly divided into three groups: normal control group, diabetes mellitus group and herbal compound group. The rats of different groups were treated with relevamt medicinals respectively by intragastrical method. In the end of 4th and 8th weeks, the rats were sacrificed and their hearts is taken out, tissue slice is taken and the morphological changes were observed under optical microscope. Detect protein expression of AngⅡ through immunohistochemistry. AngⅡ mRNA level were detected by in situ hybridization method. Statistical image analysis Results . Results In the end of 4th and 8th weeks, as compared to the normal control group, levels of myocardial AngⅡ and AngⅡ mRNA expression in myocardium, were much higher in those of diabetes mellitus group, P<0.01. Compared with diabetes mellitus group, expression level of AngⅡ and AngⅡ mRNA in herbal compound group was much lower, <0.01. Conclusion Yi-qi Yang-yin San-jie herbal compound could downregulate expression of AngⅡ and had a therapeutical effect on cardiomyopathy in DM rats.

Key words: Yi-qi Yang-yin San-jie, diabetes, cardiomyopathy, renin-angiotensin system

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