中国药物警戒 ›› 2013, Vol. 10 ›› Issue (9): 540-542.

• 药品质量与工艺研究 • 上一篇    下一篇

HPLC法测定虫草被孢菌胶囊中腺苷的含量

吴文1,刘晓鸿1,朱满芳2   

  1. 1.安徽医科大学第三附属医院,合肥市第一人民医院药学部,安徽合肥230061；
    2.安徽圣鹰药业有限公司,安徽合肥230031
  • 收稿日期:2013-05-08 修回日期:2016-03-09 出版日期:2013-09-08 发布日期:2016-03-09
  • 作者简介:吴文,女,大专,主管中药师。
  • 基金资助:
    安徽省“十二五”临床重点专科基金资助,卫科秘[2013]228号。

Using HPLC to Determine Adenosine in Cordyceps Mortierella Mycelia Capsules

WU Wen1, LIU Xiao-hong1, ZHU Man-fang2   

  1. 1.Department of Pharmacy, The Third Hospital Affiliated to Anhui Medical University, Hefei First People`s Hospital, Anhui Hefei 230061, China;
    2.Shengying Pharmacy Co.Ltd. Anhui Hefei 230031, China
  • Received:2013-05-08 Revised:2016-03-09 Online:2013-09-08 Published:2016-03-09

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摘要： 目的 建立虫草被孢菌胶囊中腺苷含量测定方法。方法 采用高效液相色谱法测定虫草被胞菌胶囊中腺苷的含量。采用Hypersil C18色谱柱（4.6 mm×250 mm,5 m）；流动相：磷酸二氢钾溶液（0.05 mol·L-1）- 甲醇（85:15）为流动相,流速1.0 mL·min-1,检测波长为260nm。结果 腺苷在1.011～50.55 g·mL-1 范围内线性关系良好,线性方程为：A=60901C-2347.7,r=1；平均回收率为100.49%（n=6,RSD=1.1%）。结论本法操作简便,不受杂质干扰,精密度好,结果 准确可靠,可用于虫草被孢菌胶囊的质量控制。

关键词: HPLC, 含量测定, 虫草被孢菌胶囊, 腺苷

Abstract: Objective To establish a method of determining adenosine in Cordyceps Mortierella Mycelia Capsules. Methods We used HPLC to determine adenosine in Cordyceps Mortierella Mycelia Capsules. In this method, HypersilC18 column(4.6 mm×250 mm,5 μm) was used with the mobile phase of KDP buffer solution(0.05 mol·L-1)-methanol(85:15). The flow rate was 1.0 mL·min-1, and the wavelength was 260 nm. Results The linear relationship of adenosine within the range of 1.011-50.55g·mL-1 is fine. The linear equation is A=60901C-2347.7, r=1, and the average recovery μrate is 100.49%(n=6, RSD=1.1%). Conclusion Using HPLC to determine adenosine in Cordyceps Mortierella Mycelia Capsules is not only easy to operate but also without the interference of impurity. It has good precision and the result is reliable. It can be used asa method in the quality control of Cordyceps Mortierella Mycelia Capsules.

Key words: HPLC, determination, Cordyceps Mortierella Mycelia Capsules, adenosine

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