中国药物警戒 ›› 2010, Vol. 7 ›› Issue (4): 193-195.

• 基础研究 •    下一篇

乳香没药及其提取物对大鼠肝毒性的实验研究

谈英1, 徐鑫2, 周昆2, 何健1, 屈彩芹2, 高秀梅2   

  1. 1三九医药股份有限公司研发事业部,深圳 518029;
    2天津中医药大学中医药研究院,天津 300193
  • 收稿日期:2016-02-19 修回日期:2016-02-19 出版日期:2010-04-08 发布日期:2016-02-19
  • 作者简介:谈英,男,硕士,主治医师,药物安全性研究与新药研发。

The Study On Hepatotoxicity of Mastic and Myrrha in Rats

TAN Ying1, XU Xin2, ZHOU Kun2, HE Jian1, QU Cai-qin2, GAO Xiu-mei2   

  1. 1R&D Department of Sanjiu Medical&Pharmacutical CO.LTD, Shenzhen 518029, China;
    2Institute of Traditional Chinese Medicine, Tianjin University of TCM, Tianjin 300193, China
  • Received:2016-02-19 Revised:2016-02-19 Online:2010-04-08 Published:2016-02-19

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摘要： 目的 比较乳香没药生药及其不同提取物对大鼠的肝脏毒性作用。方法 Wistar大鼠连续灌胃12周,分别予乳香没药生药粉、挥发油、水提物、挥发油+水提物,各给药组剂量均为3g生药/kg,同时设对照组。末次给药后禁食12 h后,腹主动脉取血进行血液生化学检测,取肝脏称重并计算肝系数,检测肝组织中的氧化应激指标丙二醛（MDA）和超氧化物歧化酶（SOD）,光镜下做肝脏病理组织学检查。结果 乳香没药生药组、水提物组、挥发油+水提物组大鼠总胆固醇（TC）明显高于对照组（P <0.01）;生药组、挥发油+水提物组大鼠白蛋白（ALB）高于对照组（P <0.05）。乳香没药生药组、水提物组、挥发油+水提物组大鼠肝脏系数明显升高（P <0.01）;挥发油组大鼠肝脏系数无明显变化。氧化应激指标检测表明,MDA除挥发油组外,其他各给药组均高于对照组（P <0.005~0.01）;SOD各给药组均没有明显影响。肝脏病理组织学检查表明,除挥发油组外,其他各给药组均出现不同程度的肝细胞空泡性病变。结论 乳香没药生药、水提物、挥发油+水提物均可造成大鼠肝脏不同程度的损伤,损伤机制可能与过氧化应激途径有关;挥发油对肝脏没有明显影响。

关键词: 乳香没药生药, 乳香没药水提物, 乳香没药挥发油, 大鼠, 肝毒性

Abstract: Objective To compare the mixture of Olibanum and Myrrha and their different extracts, so as to investigate the hepatotoxicity in rats. Methods Wistar rats were administered intragastrically for 12 weeks with Olibanum+ Myrrha crude powder, volatile oil, aqueous extract, volatile oil+aqueous extract(3g/kg) and distilled water(control group). After the end of last administration, rats were anesthesia by chloral hydrate after fasting for 12 hours. Blood was taken from abdominal aorta and blood biochemistry indices were determined. The Liver was weighted and liver coefficient was calculated, and Malondialchehyche(SOD) and Superoxide Dismutase(SOD) was tested in liver tissue also. The pathologically examination for the liver was performed with light microscopt. Results Total cholesterd(TC) in group of Olibanum+ Myrrha crude powder, aqueous extract, volatile oil+aqueous extract, were higher than that of the control group(P<0.01). Albumin(ALB) in group of Olibanum+ Myrrha crude powder, volatile oil+aqueous extract, were higher than that of the control group(P<0.05). Compared with the control group, Liver coefficient index in group of Olibanum+Myrrha crude powder, aqueous extract, volatile oil+ aqueous extract were increased significantly(P<0.01), while liver coefficient index in volatile oil group remained unchanged significantly. Malondial-dehyde(MDA) in liver tissue of the treatment group animals except volatile oil were higher than that of the control group(P<0.005~0.01).Liver histopathological examination showed that the rats of the treatment group except volatile oil were suffered from various degrees of vacuolar degeneration. Conclusion Rats in group of Olibanum and Myrrha, aqueous extract, volatile oil and aqueous extract were observed liver damage at various degrees which maybe concerned with higher oxidative stress, while no significant effect in group of volatile oil.

Key words: Olibanum and Myrrha, aqueous extract, volatile oil, rats, hepatotoxicity

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