康体瘦身方透皮成分及其干预肥胖症作用机制研究

doi:10.19803/j.1672-8629.20250941

摘要/Abstract

摘要： 目的探讨康体瘦身方治疗肥胖症的潜在活性成分及作用机制。方法采用高效液相色谱-四极杆飞行时间质谱联用技术（HPLC-Q-TOF-MS/MS）分析康体瘦身方药浴透皮实验后的透皮成分,使用PharmMapper和GeneCards等数据库筛选康体瘦身方关联靶点与肥胖相关靶点,并通过Venny筛选康体瘦身方治疗肥胖症的潜在靶点;应用String数据库与Cytoscape软件构建并分析蛋白质-蛋白质相互作用（PPI）网络,筛选核心靶点;通过基因本体（GO）和京都基因与基因组百科全书（KEGG）进行富集分析。结果共检测出21个康体瘦身方透皮成分,获得509个药物潜在靶点、1 549个肥胖症治疗潜在靶点和176个交集靶点,筛选出65个核心靶点。经网络药理学GO和KEGG富集分析,发现康体瘦身方可能通过异氨己酸（L-Norleucine）、缬氨酸（Valine）、阿魏酸（Ferulic Acid）等多成分调控AKT1、TNF、PPARγ、ESR1等多靶点,进而调节HIF-1、ROS、THR等信号通路发挥治疗肥胖症的作用。结论康体瘦身方可能通过维持糖脂代谢稳态、减轻炎症反应与氧化应激、参与食欲抑制过程,发挥其治疗肥胖的作用机制。本研究体现藏药复方“多成分、多靶点、多途径”的治疗优势,为康体瘦身方临床合理应用提供参考。

关键词: 康体瘦身方, 肥胖症, 透皮成分, 高效液相色谱-四极杆-飞行时间串联质谱, 作用机制

Abstract: Objective To study the potential transdermal active components and underlying mechanisms of Kangti Shoushen Formula (KTSF) in the treatment of obesity. Methods The transdermal components of KTSF after experimental permeation were analyzed using HPLC-Q-TOF-MS/MS. Potential targets of KTSF and obesity-related targets were obtained from the PharmMapper and GeneCards databases while the intersecting targets were identified with Venny. The STRING database and Cytoscape software were used to construct and analyze protein-protein interaction (PPI) networks and screen core targets. Finally, enrichment analysis was conducted using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Results A total of 21 transdermal components of KTSF were detected. In total, 509 putative KTSF targets and 1,549 obesity-associated targets were identified, yielding 176 overlapping targets. Subsequently, 65 core targets were prioritized. GO and KEGG enrichment analyses suggested that the anti-obesity effects of KTSF might be mediated by multiple components, including L-norleucine, valine, and ferulic acid, which acted on key targets such as AKT1, TNF, PPARγ, and ESR1, thereby modulating pathways related to HIF-1, reactive oxygen species (ROS), and thyroid hormone receptor (THR) signaling. Conclusion KTSF may deliver therapeutic effect against obesity by maintaining glucose and lipid metabolic homeostasis, alleviating inflammatory responses and oxidative stress, and participating in appetite suppression. This study reveals the therapeutic advantages of this Tibetan herbal compound in terms of its “multi-component, multi-target, multi-pathway” characteristics, providing a reference for rational clinical applications of KTSF.

Key words: Kangti Shoushen Formula, Obesity, Transdermal Components, HPLC-Q-TOF-MS/MS, Mechanism

中图分类号:

R282
R977

孙健, 杨司宇, 米玛卓嘎, 仁增, 边巴顿珠, 王榆鑫, 程浩洁, 次仁, 樊新荣. 康体瘦身方透皮成分及其干预肥胖症作用机制研究[J]. 中国药物警戒, 2026, 23(3): 259-266.

SUN Jian, YANG Siyu, MIMA Zhuoga, REN Zeng, BIANBA Dunzhu, WANG Yuxin, CHENG Haojie, CI Ren, FAN Xinrong. Transdermally Absorbed Components of Kangti Shoushen Formula and Their Mechanisms against Obesity[J]. Chinese Journal of Pharmacovigilance, 2026, 23(3): 259-266.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://zgywjj.magtechjournal.com/CN/10.19803/j.1672-8629.20250941

https://zgywjj.magtechjournal.com/CN/Y2026/V23/I3/259

参考文献

[1] YI F, Li SH, YI YL, et al.Study of Guben Huashi Jiangzhi Decoction on Feeding Mechanism of Obesity Rats Based on UHPLC-QE-MS and Network Pharmacology[J]. Traditional Chinese Drug Research and Clinical Pharmacology(中药新药与临床药理), 2023, 34(6): 806-817.
[2] World Obesity Federation.World Obesity Atlas 2025[M]. London: World Obesity Federation, 2025.
[3] ZHU XR, WEN YY, QI MT, et al.Formulation of a Self-Heating Wuwei Ganlu Hydrogel Patch and Its Anti-Inflammatory Activity[J]. Journal of Chinese Medicinal Materials(中药材), 2024, 47(2): 432-436.
[4] BAI J, LI C, YAO XH, et al.Research Progress in Regulation of Glucose and Lipid Metabolism and Intestinal Flora by Highland Barley[J]. Food Research and Development(食品研究与开发), 2025, 46(14): 198-205.
[5] HY, XIE YC, LI HL, et al.Analysis of Chemical Constituents in Tibetan Medicine Dazihao Based on UPLC-Q-Exactive Orbitrap MS[J]. Pharmacy and Clinics of Chinese Materia Medica(中药与临床), 2023, 14(2): 26-32.
[6] LI HY, SU D, BU AX, et al.Chemical Constituent Changes in Four Processing Procedures of Herbal Ephedra Based on UPLC-Q-TOF MSE and Mirror Image Contrast Analysis[J]. Journal of Chinese Mass Spectrometry Society(质谱学报), 2017, 38(6): 630-639.
[7] ZHOU X, WU HQ, LUO HT, et al.Rapid Identification of Constituents in Keke Tablets by High-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry[J]. Journal of Instrumental Analysis(分析测试学报), 2023, 42(4): 411-422.
[8] OU WJ.Research on the Chemical Constituents of the Stems and Leaves of Juniperus sibirica Burgsd.[D]. Changchun: Northeast Normal University, 2016.
[9] JI X, GE LJ, RONG H, et al.Research Progress on Chemical Constituents and Pharmacological Effects of Tibetan Medicine Rhododendron anthopogonoides Maxim.[J]. Guangxi Sciences(广西科学), 2022, 29(5): 940-948.
[10] WANG MQ.Study on Determination Method of Polyphenols and Amino Acids and Quality Analysis of Highland Barley[D]. Handan: Hebei University of Engineering, 2021.
[11] WU XY, DING Y, ZHAO CC, et al.UPLC-Q-TOF/MS-Based Analysis on the Chemical Constituents of Different Myricariae Ramulus Species[J]. Chinese Traditional Patent Medicine(中成药), 2023, 45(11): 3656-3662.
[12] LI MQ, HU XY, WANG YZ, et al.Qualitative Analysis on Chemical Constituents from Different Polarity Extracted Fractions of the Pulp and Peel of Ginger Rhizomes by Ultra-High-Performance Liquid Chromatography Coupled with Electrospray Ionization Quadrupole Time-of-Flight Tandem Mass Spectrometry[J]. Rapid Communications in Mass Spectrometry, 2021, 35(8): e9029.
[13] WANG L, FANG L, ZHAO HQ, et al.Analysis of Gingerol-Related Compounds in Fresh Ginger by HPLC-ESI-Q-TOF-MS/MS[J]. China Journal of Chinese Materia Medica(中国中药杂志), 2011, 36(24): 3467-3471.
[14] WEN N, ZHAO N, XU HX, et al.Effect of Modified Peilian Mahuang Decoction Combined with Acupoint Catgut Embedding on Simple Obesity with Stomach Heat and Dampness Obstruction Syndrome[J]. Guiding Journal of Traditional Chinese Medicine and Pharmacy(中医药导报), 2023, 29(12): 67-71.
[15] GUO LY, TAN YW, YUAN Y, et al.Study on the Mechanism of Poly-gonatum Sibiricum in Treating Obesity Based on Network Pharmacology and Experimental Validation[J]. Chemistry of Life(生命的化学), 2025, 45(3): 489-502.
[16] LIU RS.Study on the Mechanism of Luteolin and Quercetin in the Treatment of Obesity Based on Network Pharmacology and Transcri-ptome Sequencing[D]. Zhenjiang: Jiangsu University, 2022.
[17] CHEN Y, FENG YC, LIAO ZY, et al.Research Progress on Mechanisms of Obesity Regulation by Puerarin[J]. Chinese Traditional and Herbal Drugs(中草药), 2025, 56(11): 4103-4114.
[18] LI C, ZHANG H, JI CF.Research Progress on Chemical Constituents, Pharmacological Effects and Modern Application of Platycodonis Radix[J]. Chinese Pharmaceutical Journal(中国药学杂志), 2025, 60(1): 9-20.
[19] ZHANG S, YU H, LI Y.Research Progress on the Signaling Pathways of Curcumin in Obesity Treatment[J]. Acta Physiologica Sinica(生理学报), 2025, 77(6): 1047-1061.
[20] PENG DS.Anti-Obesity Mechanism of Total Saponins from Panax Japonicus[D]. Wuhan: Wuhan University, 2021.
[21] YUAN J.Mechanism of Oxytocin Improving Body Weight and Energy Metabolism in Obese Mice by Promoting Fat Differentiation[D]. Nanjing: Nanjing Medical University, 2021.
[22] DEBLON N, VEYRAT-DUREBEX C, BOURGOIN L, et al.Mechanisms of the Anti-Obesity Effects of Oxytocin in Diet-Induced Obese Rats[J]. PLoS One, 2011, 6(9): e25565.
[23] PARK JE, HAN JS.Improving the Effect of Ferulic Acid on Infla-mmation and Insulin Resistance by Regulating the JNK/ERK and NF-κB Pathways in TNF-α-Treated 3T3-L1 Adipocytes[J]. Nutrients, 2024, 16(2): 294.
[24] BERGIN R, KINLEN D, KEDIA-MEHTA N, et al.Mucosal-Associated Invariant T Cells Are Associated with Insulin Resistance in Childhood Obesity, and Disrupt Insulin Signalling via IL-17[J]. Diabetologia, 2022, 65(6): 1012-1017.
[25] YU C, NIU X, DU Y, et al.IL-17A Promotes Fatty Acid Uptake through the IL-17A/IL-17RA/p-STAT3/FABP4 Axis to Fuel Ovarian Cancer Growth in an Adipocyte-Rich Microenvironment[J]. Cancer Immunology, Immunotherapy, 2020, 69(1): 115-126.
[26] SUN Y, WANG RN, HAN YB, et al.Research Progress on the Mechanism of Traditional Chinese Medicine in the Treatment of Obesity Based on Network Pharmacology[J]. Practical Pharmacy and Clinical Remedies(实用药物与临床), 2024, 27(5): 380-386.
[27] FENG Z, DU Z, SHU X, et al.Role of RAGE in Obesity-Induced Adipose Tissue Inflammation and Insulin Resistance[J]. Cell Death & Discovery, 2021, 7(1): 1-8.
[28] ZHOU QY.Resveratrol Exerts Osteoprotective Effects on High-Fat Diet Mice through AGEs/RAGE/NF-κB Signaling Pathways[D]. Shenyang: Shenyang Medical College, 2025.
[29] LIANG B, BURLEY G, LIN S, et al.Osteoporosis Pathogenesis and Treatment: Existing and Emerging Avenues[J]. Cellular and Molecular Biology Letters, 2022, 27(1): 72.
[30] PEREZ-SERNA AA, GUZMAN-LLORENS D, DOS SANTOS RS, et al.Bcl-2 and Bcl-xL in Diabetes: Contributions to Endocrine Pancreas Viability and Function[J]. Biomedicines, 2025, 13: 223.
[31] SU Y.Exploring Lipid-Lowering Active Components in Gynostemma pentaphyllum and Their Mechanisms of Action Based on Network Pharmacology[D]. Xi’an: Shaanxi University of Science & Technology, 2022.
[32] KEREM L, LAWSON EA.The Effects of Oxytocin on Appetite Regulation, Food Intake and Metabolism in Humans[J]. International Journal of Molecular Sciences, 2021, 22(14): 7737.
[33] QI Y, LEE NJ, IP CK, et al. A Gpr-Negative Arcuate NPY Neurons Drive Feeding under Positive Energy Balance via Altering Leptin Responsiveness in POMC Neurons[J]. Cell Metabolism, 2023, 35(6): 979-995. e7.
[34] XIONG W, LIU DY.Research Progress of Hypothalamic Syndrome and Early-Onset Obesity Gene[J]. Journal of Developmental Medicine(发育医学电子杂志), 2025, 13(3): 214-218, 233.
[35] DRUCKER DJ.GLP-1 Physiology Informs the Pharmacotherapy of Obesity[J]. Molecular Metabolism, 2022, 57: 101351.