中国药物警戒 ›› 2025, Vol. 22 ›› Issue (5): 554-559.
DOI: 10.19803/j.1672-8629.20240971

• 基础与临床研究 • 上一篇    下一篇

基于LC-MS/MS的连朴饮水煎液化学成分鉴定和多成分含量测定

陈思源1,2, 张艳2, 冯雪2, 张彩娟2, 王敦方2, 刘海帆2, 刘滨2, 朱琳2, 刘鉴瑶2, 李涛2,3#, 杨伟鹏2,*   

  1. 1黑龙江中医药大学基础医学院,黑龙江 哈尔滨 150006;
    2中国中医科学院中药研究所,北京 100700;
    3中国中医科学院医学实验中心,北京 100700
  • 收稿日期:2024-12-09 出版日期:2025-05-15 发布日期:2025-05-19
  • 通讯作者: *杨伟鹏,男,博士,研究员·博导,中药药理学。E-mail：wpyang@icmm.ac.cn;#为共同通信作者。
  • 作者简介:陈思源,男,硕士,药效物质基础。
  • 基金资助:
    国家重点研发计划（2023YFC3504405）; 国家自然科学基金资助项目（82074328）; 中国中医科学院科技创新工程项目（CI2021B015、CI2021A04604）

Identification of Chemical Constituents and Determination of Multi-Component Content of Lianpo Drinking Water Decoction Based on LC-MS/MS Technology

CHEN Siyuan1,2, ZHANG Yan2, FENG Xue2, ZHANG Caijuan2, WANG Dunfang2, LIU Haifan2, LIU Bin2, ZHU Lin2, LIU Jianyao2, LI Tao2,3#, YANG Weipeng2,*   

  1. 1Basic Medical College, Heilongjlang University of Chinese Medicine, Harbin Heilongjlang150006, China;
    2Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;
    3Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Received:2024-12-09 Online:2025-05-15 Published:2025-05-19

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摘要： 目的 采用HPLC-LTQ-Orbitrap MS/MS技术鉴定连朴饮水煎液的化学成分,运用HPLC-QQQ MS/MS对连朴饮水煎液的11种成分进行含量测定。方法 化学成分鉴定和定量均选用ACQUITY UPLC BEH C18色谱柱（2.1 mm×100 mm,1.7 µm）,0.1%甲酸水溶液（A）-乙腈（B）为流动相,梯度洗脱。结果 从连朴饮水煎液中鉴定出50种化学成分,包括15种有机酸类、10种生物碱类、6种萜类和5种黄酮类等,其中黄连碱、葫芦巴碱、染料木素等11种成分通过对照品比对确认;建立了连朴饮水煎液中黄连碱、葫芦巴碱、染料木素、巴马汀、小檗碱、阿魏酸、栀子苷、厚朴酚、厚朴酚、大豆苷元、琥珀酸同时定量的HPLC-QQQ MS/MS方法。各成分分析浓度范围内线性关系良好（R>0.999）,平均加样回收率90.78%~115.10%,重复性试验的相对标准偏差（RSD）均小于5.5%。结论 该研究鉴定了连朴饮水煎液的化学成分,建立了同时测定连朴饮水煎液中11种成分含量的方法,该方法灵敏、准确、快速,为进一步建立连朴饮水煎液的质量控制方法提供了参考。

关键词: 连朴饮, 化学成分, 黄连碱, 葫芦巴碱, 染料木素, 含量测定, HPLC-LTQ-Orbitrap MS/MS, HPLC-QQQ MS/MS

Abstract: Objective To identify the chemical constituents of Lianpo decoction, and to establish a method for simultaneous determination of the contents of 11 active substances in the decoction using HPLC-QQQ MS/MS. Methods The ACQUITY UPLC BEH C18 column (2.1 mm×100 mm, 1.7 µm) with 0.1% aqueous formic acid (A)-acetonitrile (B) as the mobile phase and under gradient elution was used for the identification and quantification of the chemical components. Results Fifty chemical components were identified from the Lianpo decoction, including 15 organic acids, 10 alkaloids, 6 terpenes and 5 flavonoids. Among them, 11 components such as coptisine, trigonelline and genistein were confirmed through a comparison with reference standards. An HPLC-QQQ MS/MS method was developed for simultaneous quantification of coptisine, trigonelline, genistein, palmatine, berberine, ferulic acid, geniposide, honokiol, magnolol, daidzein, and succinic acid in the decoction. The concentration ranges of the components showed a good linearity (R>0.999), the average spiked recoveries ranged from 90.78% to 115.10%, and the relative standard deviations (RSDs) of the repeatability tests were all less than 4%. Conclusion The study has identified the chemical constituents of Lianpo decoction, and established a method for simultaneous determination of 11 components. This method is sensitive, accurate and rapid, which can help establish more methods for quality control of Lianpo decoction.

Key words: Lianpo Decoction, Chemical Constituents, Coptisine, Trigonelline, Genistein, Content Determination, HPLC-LTQ-Orbitrap MS/MS, HPLC-QQQ MS/MS

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