火木层孔菌桑黄对MPP+诱导SH-SY5Y细胞凋亡的影响

中国药物警戒 ›› 2018, Vol. 15 ›› Issue (6): 325-328.

火木层孔菌桑黄对MPP⁺诱导SH-SY5Y细胞凋亡的影响

郭姗姗¹, WolfDieterRausch²

1中国中医科学院中药研究所,北京100029;
2维也纳兽医大学医学生物化学学院,维也纳A-1210

收稿日期:2018-08-03 修回日期:2018-08-03 出版日期:2018-06-15 发布日期:2018-08-03
作者简介:郭姗姗,女,博士,副研究员,中药药理。
基金资助:
中国中医科学院中药研究所自主选题项目（ZXKT17011）：药用真菌桑黄对结肠癌细胞增殖的抑制作用研究

Effects of Phellinus igniarius on MPP⁺-induced Apoptosis in SH-SY5Y Cell Line

GUO Shan-shan¹, Wolf Dieter Rausch²

1 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;
2 Institute for Medical Biochemistry, University for Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria

Received:2018-08-03 Revised:2018-08-03 Online:2018-06-15 Published:2018-08-03

摘要/Abstract

摘要： 目的评价火木层孔菌桑黄的水提物对MPP⁺诱导SH-SY5Y细胞凋亡的影响。方法采用PI染色分析细胞凋亡率,DAPI染色观察细胞核的形态变化,JC-1染色检测线粒体膜电位的变化,C-DCDHF-DA染色观察ROS生成的变化。结果 MPP⁺诱导后SH-SY5Y细胞凋亡率明显升高,线粒体膜电位明显下降,ROS生成明显升高;火木层孔菌桑黄作用后能明显降低细胞凋亡率,其中20、10 μg·mL^-1剂量组与MPP⁺组比较具有显著性差异;其对细胞凋亡后细胞核的形态变化具有一定的改善作用;其可以提升线粒体膜电位,其中20、10、5、2.5 μg·mL^-1剂量组与MPP⁺组比较具有显著性差异;其对ROS生成具有明显的抑制作用,其中20、10、5 μg·mL^-1剂量组与MPP⁺组比较具有显著性差异。结论火木层孔菌桑黄水提物对MPP⁺诱导的SH-SY5Y细胞凋亡具有明显的抑制作用。

关键词: 桑黄, 火木层孔菌, 细胞凋亡, SH-SY5Y细胞, 线粒体膜电位

Abstract: Objective To evaluate the inhibitory effects of water extract of Phellinus igniarius on MPP⁺-induced apoptosis in SH-SY5Y cell line. Methods The relative apoptosis rate was analyzed by PI staining. Morphological changes of nucleus were observed by DAPI staining. Changes of mitochondrial membrane potential were detected by JC-1 staining. ROS production was measured by C-DCDHF-DA staining. Results The relative apoptosis rate of SH-SY5Y cells increased obviously after MPP⁺ induction, the mitochondrial membrane potential decreased remarkably, and ROS production increased significantly. Compared with the MPP⁺ control group, the apoptosis rates were significantly decreased by Phellinus igniarius at doses of 20 and 10 μg·mL^-1 respectively. The morphological changes of cell nuclei after apoptosis were reversed by Phellinus igniarius. Phellinus igniarius treatment (20, 10, 5, 2.5 μg·mL^-1) dramatically increased relative mitochondrial membrane potential compared with MPP⁺ control respectively. Compared with the MPP⁺ control, Phellinus igniarius treatment (20, 10, 2.5 μg·mL^-1) significantly decreased relative ROS formation respectively. Conclusion The water extract of Phellinus igniarius showed significant inhibitory effect on SH-SY5Y cells apoptosis induced by MPP⁺.

Key words: Phellinus, Phellinus igniarius, apoptosis, SH-SY5Y cell, mitochondrial membrane potential

中图分类号:

R965

郭姗姗, WolfDieterRausch. 火木层孔菌桑黄对MPP⁺诱导SH-SY5Y细胞凋亡的影响[J]. 中国药物警戒, 2018, 15(6): 325-328.

GUO Shan-shan, Wolf Dieter Rausch. Effects of Phellinus igniarius on MPP⁺-induced Apoptosis in SH-SY5Y Cell Line[J]. Chinese Journal of Pharmacovigilance, 2018, 15(6): 325-328.

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