EGFL7单克隆抗体体外抑制小鼠ACTH垂体腺瘤AtT-20细胞增殖和侵袭的作用研究

中国药物警戒 ›› 2018, Vol. 15 ›› Issue (6): 321-324.

• 基础与临床研究 • 下一篇

EGFL7单克隆抗体体外抑制小鼠ACTH垂体腺瘤AtT-20细胞增殖和侵袭的作用研究

刘潜, 王红云, 李丹, 龚磊, 张亚卓^*

北京市神经外科研究所,北京 100050

收稿日期:2018-08-03 修回日期:2018-08-03 出版日期:2018-06-15 发布日期:2018-08-03
通讯作者: 张亚卓,男,主任医师,教授·博导,中枢神经系统肿瘤的基础和临床研究。E-mail：zyz2004520@yeah.net
作者简介:刘潜,女,博士,中枢神经系统肿瘤及分子药理研究。
基金资助:
国家自然科学基金（81502154）：EGFL7调控EGFR介导的Akt/MAPK通路促进垂体腺瘤侵袭及机制研究

Effects of EGFL7-blocking Monoclone Antibody Inhibiting Proliferation and Invasion of Mouse Adrenocorticotropic Hormone Pituitary Adenoma AtT-20 Cells in vitro

LIU Qian, WANG Hong-yun, LI Dan, GONG Lei, ZHANG Ya-zhuo^*

Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China

Received:2018-08-03 Revised:2018-08-03 Online:2018-06-15 Published:2018-08-03

摘要/Abstract

摘要： 目的观察EGFL7单克隆抗体对小鼠ACTH垂体腺瘤AtT-20细胞增殖和侵袭的影响。方法 MTS试验检测不同浓度EGFL7单克隆抗体处理AtT-20细胞24、48、72 h后细胞增殖活力。ELISA检测不同浓度的EGFL7单克隆抗体处理AtT-20细胞72 h后细胞上清中ACTH的分泌水平。Real-time PCR检测AtT-20细胞经EGFL7单克隆抗体处理24 h后细胞中侵袭相关基因E-cadherin、snail、vimentin的mRNA表达水平。结果 EGFL7单克隆抗体能够有效抑制AtT-20细胞增殖,并呈现良好的时间依赖性与浓度依赖性;同时能够显著降低AtT-20细胞分泌ACTH,呈现良好的剂量依赖作用。Real-time-PCR结果表明EGFL7单克隆抗体能够显著抑制AtT-20细胞中侵袭相关基因E-cadherin、snail、vimentin的mRNA表达。结论 EGFL7单克隆抗体可以显著抑制AtT-20细胞的增殖和侵袭。

关键词: EGFL7, 单克隆抗体, AtT-20细胞, 细胞增殖, 细胞侵袭

Abstract: Objective To study the effects of EGFL7-blocking monoclone antibody inhibiting the proliferation and invasion of adrenocorticotropic hormone (ACTH) pituitary adenoma AtT-20 cells in vitro. Methods After treated with different concentrations of EGFL7-blocking monoclone antibody for 24, 48, 72 h, the proliferation of AtT-20 cells was determined by MTS. Simultaneously, ACTH secretion of AtT-20 cells by ELISA and the mRNA expression of invasion related genes such as E-cadherin, snail, vimentin were detected by Real-time PCR. Results EGFL7-blocking monoclone antibody could effectively inhibit the proliferation and ACTH secretion of AtT-20 cells in a dose-dependent and time-dependent manner. Also, EGFL7-blocking monoclone antibody significant down-regulated the mRNA level of E-cadherin、snail, vimentin in AtT-20 cells. Conclusion EGFL7-blocking monoclone antibody could inhibit mouse pituitary adenoma AtT-20 cells proliferation and invasion.

Key words: EGFL7, monoclone antibody, AtT-20 cells, proliferation, invasion

中图分类号:

R965

刘潜, 王红云, 李丹, 龚磊, 张亚卓. EGFL7单克隆抗体体外抑制小鼠ACTH垂体腺瘤AtT-20细胞增殖和侵袭的作用研究[J]. 中国药物警戒, 2018, 15(6): 321-324.

LIU Qian WANG Hong-yun LI Dan GONG Lei, ZHANG Ya-zhuo. Effects of EGFL7-blocking Monoclone Antibody Inhibiting Proliferation and Invasion of Mouse Adrenocorticotropic Hormone Pituitary Adenoma AtT-20 Cells in vitro[J]. Chinese Journal of Pharmacovigilance, 2018, 15(6): 321-324.

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EGFL7单克隆抗体体外抑制小鼠ACTH垂体腺瘤AtT-20细胞增殖和侵袭的作用研究

Effects of EGFL7-blocking Monoclone Antibody Inhibiting Proliferation and Invasion of Mouse Adrenocorticotropic Hormone Pituitary Adenoma AtT-20 Cells in vitro

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