中国药物警戒 ›› 2017, Vol. 14 ›› Issue (4): 193-195.

• 基础与临床研究 •    下一篇

双环醇在α-萘异硫氰酸酯诱导的小鼠胆汁淤积性黄疸的作用观察

刘宁, 刘威, 张丹, 鲍秀琦, 孙华   

  1. 中国医学科学院/北京协和医学院药物研究所,北京 100050
  • 收稿日期:2017-07-05 修回日期:2017-07-05 出版日期:2017-04-20 发布日期:2017-07-05
  • 通讯作者: 孙华,博士,副研究员,肝脏生化药理学。E-mail：sunhua@imm.ac.cn
  • 作者简介:刘宁,女,在读硕士,肝脏生化药理学。
  • 基金资助:
    国家自然科学基金面上项目（81173073）

Effects of Bicyclol on α-naphthalene Isothiocyanate-induced Liver Injury in Mice

LIU Ning, LIU Wei, ZHANG Dan, BAO Xiu-qi, SUN Hua   

  1. Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
  • Received:2017-07-05 Revised:2017-07-05 Online:2017-04-20 Published:2017-07-05

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摘要： 目的 观察双环醇对α-萘异硫氰酸酯（ANIT）致小鼠淤积性黄疸的作用。方法 ICR小鼠连续灌胃给予双环醇200mg·kg-1 5天,每天给药1次,在第3天给药后2 h单次灌胃给予ANIT 110 mg·kg-1,观察动物状态并继续给药。双环醇末次给药前动物禁食不禁水12 h,末次给药后2 h处理动物,计算肝脾指数,制备血清,全自动生化分析仪检测血清ALT、AST、ALP、TBIL、DBIL和TBA水平,制备10%肝匀浆,测定肝组织MDA和SOD含量。结果 ANIT可引起小鼠显著毒性和肝组织损伤,动物出现死亡,脾脏指数降低,血清转氨酶及胆红素、胆汁酸等含量显著升高,肝组织MDA含量升高,SOD降低。双环醇200 mg·kg-1连续给药5次,能降低动物死亡率,改善脾脏指数,但对ANIT致小鼠血清肝功能生化指标升高无降低作用,进一步增大肝脏指数,且有进一步加重的趋势。结论 双环醇有加重ANIT诱导的小鼠淤积性黄疸的作用,需引起注意。

关键词: α, -萘异硫氰酸酯, 淤积性黄疸, 双环醇

Abstract: Objective To observe the effects of bicyclol on α-naphthalene isothiocyanate (ANIT) -induced liver injury in ICR mice. Methods ICR mice were given intragastric administration bicyclol 200 mg·kg-1 for five days continuously, oncedaily. Two hours after the third administration, the mice were treated with ANIT 110 mg·kg-1 to establish the liver damage model. All animals were killed on 2 h after the fifth bicyclol administration. The activities of ALT, AST, ALP, TBIL,DBIL and TBA in serum were measured by automatic chemistry analyzer. 10% liver homogenate were prepared to test the MDA and SOD. Results ANIT 110 mg·kg-1 could induce significant liver damage, the mortality rate of mice increased and the levels of serum ALT, AST, ALP, ALB, TBIL, DBIL and TBA were significantly increased. 200 mg·kg-1 bicyclol had no even aggravating effects on the elevated level of the blood biochemical indexes as well as MDA and SOD. Conclusion Bicyclol aggravates ANIT-induced liver injury in mice probably and this should catch our attentions.

Key words: α-naphthalene isothiocyanate, liver injury, bicyclol.

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