中国药物警戒 ›› 2017, Vol. 14 ›› Issue (10): 603-606.

• 基础与临床研究 • 上一篇    下一篇

何首乌不同炮制品醇提物对小鼠急性毒性实验比较研究

赵新妹1,2, 李晓宇1,2, 孙蓉3*, 黄娜娜4, 王燕青1, 郭欣1, 蔡涛涛1, 吕莉莉2   

  1. 1.山东中医药大学，山东济南250355;
    2.山东省中医药研究院，山东济南250014;
    3.山东大学齐鲁医学部，山东济南250012;
    4.山东大学第二医院，山东济南250033
  • 收稿日期:2017-09-25 修回日期:2017-11-30 出版日期:2017-10-20 发布日期:2017-11-30
  • 通讯作者: 孙蓉，女，博士，教授·博导，中药药理与毒理。E-mail：sunrong107@163.com
  • 作者简介:赵新妹，女，在读硕士，中药药理与毒理。
  • 基金资助:
    国家公益性行业科研专项经费项目（201507004-4-4）：何首乌炮制和工艺减毒及合理用药对策研究；国家重点基础研究发展计划（973）中医基础理论专项资助项目（2009CB522802）；山东省中医药科技发展计划项目（2013-120）：吴茱萸肝毒性氧化损伤路径与凋亡机制研究。

Study on Acute Toxicity of Different Processed Ethanol Extract of Polygoni Multiflori in Mice

ZHAO Xin-mei1,2, LI Xiao-yu1,2, SUN Rong3* HUANG Na-na4 WANG Yan-qing1, GUO Xin1, CAI Tao-tao1, LV Li-li2,   

  1. 1.Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China;
    2.Shandong Academy of Chinese Medicine, Shandong Jinan 250014, China;
    3.Cheeloo Health Science Center, Shandong University, Shandong Jinan 250012, China;
    4.The Second Hospital of Shandong University, Shandong Jinan 250033, China
  • Received:2017-09-25 Revised:2017-11-30 Online:2017-10-20 Published:2017-11-30

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摘要： 目的 比较何首乌不同炮制品醇提物对小鼠急性毒性的大小。方法 采用60%乙醇提取的方法，按照《中华人民共和国药典》2015年版和上海、北京、安徽、浙江与河南地方炮制规范，分别制备何首乌黑豆煮组，黑豆汁蒸6 h组，隔水炖组，清蒸组，黑豆汁蒸18 h组的五种炮制品，运用小鼠经典急性毒性的半数致死量LD50、最大耐受量MTD、最大给药量MFD实验方法，分别进行何首乌不同炮制品醇提物对小鼠急性毒性比较研究。结果 何首乌生品及不同炮制品醇提物的急毒结果均为最大耐受量（MTD），毒性表现主要为俯卧、怠动，死亡前表现为全身颤抖、呼吸困难，并且在药后5h内出现死亡。生品组、黑豆汁蒸6 h组、黑豆汁蒸18 h组、黑豆煮组、隔水炖组、清蒸组MTD值分别为101.08、140.76、227.52、122.04、141.48和163.88 g·kg-1，分别相当于临床70.0 kg成人每kg体重日用量的1 175.3、823.2、1 330.5、713.7、827.4与958.4倍。结论 何首乌不同炮制品醇提物毒性强度依次为：生品组>黑豆煮组>黑豆汁蒸6组>隔水炖组>清蒸组>黑豆汁蒸18 h组，提示何首乌不同炮制方式可影响其醇提物的急性毒性大小。

关键词: 何首乌不同炮制品, 醇提物, 急性毒性

Abstract: Objective To compare the acute toxicity of different processed ethanol extracts of Polygoni Multiflori. Methods Chinese Pharmacopoeia 2015 Edition and Shanghai, Beijing, Anhui, Zhejiang and Henan provincial specification of Chinese materia medica was provided for preparing five processed products. They are Bean-processing, Bean juice-6 h, Stew, Steamed and Bean juice-18 h respectively. According to the classical method (median lethal dose LD50, maximum tolerated dose MTD, maximum dose MFD) of acute toxicity, and compare the acute toxicity of raw and different processed products from Polygoni Multiflori in mice. Results The raw and different processed products from Polygoni Multiflori had MTD value. The main toxicities of Polygoni Multiflori were lie prostrate, lazy movement, and trembling, and dyspnea before death. The MTD of raw, Bean-processing, Bean juice-6 h, Stew, Steamed and Bean juice-18 h was 101.08, 140.76, 227.52, 122.04, 141.48, 163.88 g·kg-1 respectively, which was respectively equal to 1 175.3, 823.2, 1 330.5, 713.7, 827.4, and 958.4 times of 70 kg people's daily dried medicinal herb expenses. Conclusion The raw and different processed products from Polygoni Multiflori can cause certain acute toxicity effect on normal mice, the size order is raw > Bean-processing > Bean juice-6 h > Stew > Steamed > Bean juice-18 h. Different ways of Polygoni Multiflori can affect its toxicity. It was suggested that the different processing methods of Polygoni Multiflori could affect the toxicity of its alcohol extracts.

Key words: different processed products from Polygoni Multiflori, alcohol extraction components, acute toxicity

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