绝经后妇女长期低剂量激素替代疗法中外周血内吗啡肽1和2的分析研究

中国药物警戒 ›› 2016, Vol. 13 ›› Issue (4): 212-215.

绝经后妇女长期低剂量激素替代疗法中外周血内吗啡肽1和2的分析研究

王涛^1,2,任志丽²,纪超²,杨楠²,刘雁勇²,左萍萍²

1国家食品药品监督管理总局药品评价中心,北京100045;
2 中国医学科学院基础医学研究所药理室,北京100005

收稿日期:2016-02-18 修回日期:2016-06-30 出版日期:2016-04-20 发布日期:2016-06-30
通讯作者: 左萍萍,女,博士,研究员,衰老与内分泌机制研究。E-mail: pingping_zuo@126.com
作者简介:王涛,男,博士,副研究员,神经药理学研究及药品不良反应监测与评价。
基金资助:
北京市自然科学基金青年基金项目（7154211）; 2014年北京市优秀人才培养资助（青年骨干项目）; 2015年神经再生修复研究北京市重点实验室课题（ 2015SJZS02）。

Analysis of Endomorphin-1 and Endomorphin-2 in Peripheral Blood of Postmenpausal Women with Long-term Low-dose Hormone Replacement Therapy

WANG Tao^1,2, REN Zhi-li², JI Chao², YANG Nan², LIU Yan-yong², ZUO Ping-ping²

1Center for Drug Reevaluation, CFDA, Beijing 100045, China;
2Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, China

Received:2016-02-18 Revised:2016-06-30 Online:2016-04-20 Published:2016-06-30

摘要/Abstract

摘要： 目的在长期低剂量激素替代疗法（HRT）下,观察绝经后妇女外周血中内吗啡肽1（EM^-1）和内吗啡肽2（EM^-2）的变化,并对内吗啡呔与雌激素进行相关性分析。方法研究对象系北京协和医院已绝经女职工共169人,全员根据年龄共分为4组,包括50~59岁, 60~64岁, 65~69岁, 70岁以上,每年龄段内均包括HRT组和对照组。用酶联免疫反应测试仪测定受试者血浆性激素水平,用放射免疫法检测血浆内EM^-1和EM^-2的含量。结果各年龄段HRT治疗组E2的水平均显著高于对照组（P<0.05）;各年龄段HRT治疗组EM^-1和EM^-2的水平均低于对照组,且全体受试者血浆雌激素E2的水平与EM^-1具有Pearson’s相关性。结论首次观察了在长期低剂量HRT疗法下绝经后妇女外周血中内吗啡肽1和2的变化,该结果对如何保护绝经后妇女的脑认知具有一定的临床意义。

关键词: 激素替代疗法, 内吗啡肽1, 内吗啡肽2

Abstract: Objective To investigate the levels of EM^-1 and EM^-2 in peripheral blood with long-term low dose HRT therapy and analyze the relationship between endomorphins and estradiol. Methods 169 female medical staff in Peking Union Medical College Hospital were included in our study. Based on age, participants were divided into 4 groups, including 50~59 , 60~64 , 65~69 and over 70 years old , HRT group and control group were included in each age group. Plasma hormones including estradiol (E2), progesterone (P) and testosterone (T) were detected by using Bio-RAD 550 Model ELISA(enzyme-linked immunosorbent assay) device, and levels of EM^-1 and EM^-2 were examined by radioimmunoassay methods. Results Compared to control group at each age, plasma estrodiol (E2) levels obviously increased in HRT groups (P<0.05). The levels of EM^-1 and EM^-2 in HRT group of each age decreased compared to control group, meanwhile, pearson correlation analysis showed that the level of endomorphin^-1 (EM^-1) on all subjects was correlated with their individual estradiol(P<0.01). Conclusion This is the first observation of the relationship between endomorphins and estradiol in a long-term low-dose HRT therapy, which providing clinical significance of protecting brain cognition in postmenpausal women.

Key words: hormone replacement therapy(HRT), endomorphin-1(EM-1), endomorphin-2(EM-2)

中图分类号:

R969.4

王涛,任志丽,纪超,杨楠,刘雁勇,左萍萍. 绝经后妇女长期低剂量激素替代疗法中外周血内吗啡肽1和2的分析研究[J]. 中国药物警戒, 2016, 13(4): 212-215.

WANG Tao, REN Zhi-li, JI Chao, YANG Nan, LIU Yan-yong, ZUO Ping-ping. Analysis of Endomorphin-1 and Endomorphin-2 in Peripheral Blood of Postmenpausal Women with Long-term Low-dose Hormone Replacement Therapy[J]. Chinese Journal of Pharmacovigilance, 2016, 13(4): 212-215.

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