中国药物警戒 ›› 2016, Vol. 13 ›› Issue (2): 65-69.

• 基础研究 •    下一篇

丹参联合运动对老年血瘀症大鼠模型的影响及机制研究

贾景景1， 2， 毛鑫2， 3， 陈影2， 付英杰4， 韩淑娴2， 王智民2， 廖福龙2， 游云2*   

  1. 1河南中医药大学药学院，河南 郑州450046;
    2.中国中医科学院中药研究所，北京100700;
    3.北京中医药大学药学院，北京100102;
    4.帕瑞医学科技（北京）有限公司，北京100012
  • 收稿日期:2016-03-14 修回日期:2016-03-14 出版日期:2016-02-08 发布日期:2016-03-14
  • 通讯作者: 游云,女,博士,研究员·硕导,中药药理学。E-mail: youyunrice@126.com
  • 作者简介:贾景景，女，在读硕士，中药学。
  • 基金资助:
    国家自然科学基金项目（81274006）

Effects of Salvia Miltiorrhiza and Exercise on Blood Stasis Aged Rat Model and Its Mechanism

JIA Jing-jing1, 2, MAO Xin2, 3, CHEN Ying2, FU Ying-jie4, HAN Shu-xian2, WANG Zhi-min2, LIAO Fu-long2, YOU Yun2, *   

  1. 1 School of Pharmacy, Henan University of Traditional Chinese Medicine, Henan Zhengzhou 450046, China;
    2 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;
    3.School of Pharmacy, Beijing University of Traditional Chinese Medicine, Beijing 100102, China;
    4 .Perimed China Ltd, Beijing 100012, China
  • Received:2016-03-14 Revised:2016-03-14 Online:2016-02-08 Published:2016-03-14

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摘要： 目的研究丹参联合运动对老年血瘀症大鼠模型的影响及其可能的交互作用。方法D-半乳糖皮下注射，建立老年血瘀大鼠模型。采用3×3析因设计分组，将模型动物随机分为9组，分别给予丹参（0、1.5、3 g·kg-1）及游泳（0、20、40 min）处理。持续7周后，采用激光散斑血流监测视频系统检测大鼠下肢微循环血流灌注量的变化，测定下肢压力后反应性充血（PORH）。腹主动脉采血测定血小板聚集率和纤维蛋白原含量，取血清测定超氧化物歧化酶（SOD）和丙二醛（MDA）。结果丹参与运动均可提高模型大鼠下肢微循环灌注量（P<0.01， P<0.05），且二者有交互作用（P<0.05）。丹参可显著提高模型大鼠下肢充血速率（P<0.01）。丹参与运动均可明显降低模型大鼠血小板聚集率（P<0.05， P<0.05），且均可降低模型大鼠FIB含量（P<0.01， P<0.01），且二者有交互作用（P<0.01）。运动可显著提高模型大鼠血清中SOD的含量（P<0.05）。丹参和运动均可降低模型大鼠血清中MDA含量（P<0.05， P<0.01）。结论丹参联合适当运动可降低血小板聚集率、FIB、MDA，并提高SOD，从而提高老年血瘀大鼠微循环灌注量，改善微循环障碍。

关键词: 丹参, 运动, 微循环, 血管内皮功能

Abstract: ObjectiveTo investigate the effects and possible interactions of Salvia miltiorrhiza combined exercise on blood stasis in aged rats. MethodsBlood stasis aged rat model was established by subcutaneously injecting D- galactose. 3×3 factor experimental design was used. Rats were randomly divided into nine groups, namely different levels of Salvia miltiorrhiza (0, 1.5, 3 g·kg-1) and different levels of swimming (0, 20, 40 min). After seven weeks, Laser Doppler blood flow imaging system was used to monitor the change of microcirculation blood flow of rat lower limbs and detect the post-occlusion reactive hyperaemia (PORH) of rat lower limbs. The plasma samples of rats were collected to determine the content of platelet aggregation and fibrinogen (FIB). The serum samples were collected to detect the level of superoxide dismutase (SOD) and malondialdehyde (MDA). ResultsBoth Salvia miltiorrhiza and exercise could improve the microcirculation blood flow of model rat lower limbs (P<0.01, P<0.05) and they had interaction (P<0.05). Salvia miltiorrhiza could significantly enhance the hyperemia rate of model rat lower limbs (P<0.01). Both Salvia miltiorrhiza and exercise could reduce the rate of platelet aggregation of model rats (P<0.05, P<0.05), and lower the content of FIB of model rats (P<0.01, P<0.01) and they had interaction (P<0.01). Exercise could increase the level of SOD in model rats (P<0.05). Both Salvia miltiorrhiza and exercise could decrease content of MDA of model rats (P<0.01, P<0.05). ConclusionSalvia miltiorrhiza combined appropriate exercise could improve the microcirculatory disturbances of blood stasis aged rats through reducing the rate of platelet aggregation and the content of FIB and MDA, increasing the level of SOD and the microcirculation blood flow.

Key words: Salvia miltiorrhiza, exercise, microcirculation, vascular endothelial function

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