中国药物警戒 ›› 2016, Vol. 13 ›› Issue (2): 70-73.

• 基础研究 • 上一篇    下一篇

金铁锁去皮根、带皮根、根皮水煎液对小鼠急性毒性的实验研究

吴玟萱1， 郭建友2， 王谦1， 张北月1， 王胜男1， 马晓杰1， 郑志全1， 石晋丽1*   

  1. 1北京中医药大学，北京 100102;
    2.中国科学院心理研究所，北京 100101
  • 收稿日期:2016-03-14 修回日期:2016-03-14 出版日期:2016-02-08 发布日期:2016-03-14
  • 通讯作者: 石晋丽,女,教授,博士生导师,中药质量标准研究。E-mail:shijl@vip.sina.com。
  • 作者简介:吴玟萱，女，在读硕士，中药质量标准研究。
  • 基金资助:
    国家药典委员会“国家药品提高暨2015版药典科研任务”项目(金铁锁药材及饮片质量标准的修订)

Comparison Study on Acute Toxicity of the Aqueous Solution from Radix, Radix with Cortex and Cortex of Psammosilene Tunicoides

WU Min-xuan1, GUO Jian-you2, WANG Qian1, ZHANG Bei-yue1, WANG Sheng-nan1, MA Xiao-jie1, ZHENG Zhi-quan1, SHI Jin-li1, *   

  1. 1Beijing University of Chinese Medicine, Beijing 100102, China;
    2. Institute of Psychology, Chinese Academy of Science, Beijing 100101, China
  • Received:2016-03-14 Revised:2016-03-14 Online:2016-02-08 Published:2016-03-14

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摘要： 目的测定比较金铁锁去皮根、带皮根、根皮三样品水煎液的急性毒性，为临床安全合理用药提供依据。方法采用经典的急性毒性实验方法，进行金铁锁三样品水煎液的小鼠急性毒性实验比较，记录死亡情况、毒性症状、体重变化、死亡小鼠高剂量进行组织病理观察，用Bliss法计算半数致死量（LD50）。结果金铁锁去皮根、带皮根、根皮水煎液的LD50值（95%可信限）分别为: 4.638 2 (4.004 6~5.439 6) g·kg-1、4.847 1 (4.325 1~5.450 8) g·kg-1、6.403 2(5.720 7~7.210 3) g·kg-1（生药量），毒性靶器官主要为肺、脾、胃。结论金铁锁不同部位水煎液的LD50值:根皮>带皮根>去皮根，差异较小。其毒性体内过程、毒性机制等尚待进一步研究。

关键词: 金铁锁, 水煎液, 急性毒性

Abstract: ObjectiveTo compare the acute toxicity of the aqueous solution from Psammosilenes radix, Psammosilenes radix with cortex and Psammosilenes cortex, in order to provide a basis for safe and reasonable medication in clinic. MethodsThree samples of Psammosilenes tunicoides were prepared and compared in accordance with classical acute toxicity test. The toxic symptom was observed, and the death and the change of body weight were recorded. The pathological morphology parameters of dead mice with high dose were performed. The experimental data are calculated LD50 with the Bliss method. ResultsThe LD50 (the 95% confidence interval) of Psammosilenes radix, Psammosilenes radix with cortex, Psammosilenes cortex are respectively 4.638 2 (4.004 6~5.439 6) g·kg-1, 4.847 1 (4.325 1~5.450 8) g·kg-1, 6.403 2 (5.720 7~7.210 3) g·kg-1 (crude drug dose). The main toxic target organs are lung, spleen and stomach. ConclusionThe value of LD50 of different fractions in Psammosilenes tunicoides is: Psammosilenes cortex >Psammosilenes radix with cortex> Psammosilenes radix. However, it has slight difference. The process of toxicity in vivo and toxicity mechanism need to be further studied.

Key words: Psammosilenes tunicoides, aqueous solution, acute toxicity

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